Cre-loxP-controlled periodic Aurora-A overexpression induces mitotic abnormalities and hyperplasia in mammary glands of mouse models

Dongwei Zhang, Toru Hirota, Tomotoshi Marumoto, Michio Shimizu, Naoko Kunitoku, Takashi Sasayama, Yoshimi Arima, Liping Feng, Misao Suzuki, Motohiro Takeya, Hideyuki Saya

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Aurora-A, a serine/threonine mitotic kinase, was reported to be overexpressed in various human cancers, and its overexpression induces aneuploidy, centrosome amplification and tumorigenic transformation in cultured human and rodent cells. However, the underlying mechanisms and pathological settings by which Aurora-A promotes tumorigenesis are largely unknown. Here, we created a transgenic mouse model to investigate the involvement of Aurora-A overexpression in the development of mammary glands and tumorigenesis using a Cre-loxP system. The conditional expression of Aurora-A resulted in significantly increased binucleated cell formation and apopiosis in the mammary epithelium. The surviving mammary epithelial cells composed hyperplastic areas after a short latency. Induction of Aurora-A overexpression in mouse embryonic fibroblasts prepared from the transgenic mice also led to aberrant mitosis and binucleated cell formation followed by apoptosis. The levels of p53 protein were remarkably increased in these Aurora-A-overexpressing cells, and the apoptosis was significantly suppressed by deletion of p53. Given that no malignant tumor formation was found in the Aurora-A-overexpressing mouse model after a long latency, additional factors, such as p53 inactivation, are required for the tumorigenesis of Aurora-A-overexpressing mammary epithelium. Our findings indicated that this mouse model is a useful system to study the physiological roles of Aurora-A and the genetic pathways of Aurora-A-induced carcinogenesis.

Original languageEnglish
Pages (from-to)8720-8730
Number of pages11
JournalOncogene
Volume23
Issue number54
DOIs
Publication statusPublished - 2004 Nov 18
Externally publishedYes

Fingerprint

Human Mammary Glands
Hyperplasia
Carcinogenesis
Breast
Transgenic Mice
Epithelium
Neoplastic Cell Transformation
Apoptosis
Centrosome
Protein-Serine-Threonine Kinases
Aneuploidy
Mitosis
Rodentia
Neoplasms
Fibroblasts
Epithelial Cells
Proteins

Keywords

  • Aurora-A
  • Cre-loxP
  • Mammary gland
  • Mitosis
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Cre-loxP-controlled periodic Aurora-A overexpression induces mitotic abnormalities and hyperplasia in mammary glands of mouse models. / Zhang, Dongwei; Hirota, Toru; Marumoto, Tomotoshi; Shimizu, Michio; Kunitoku, Naoko; Sasayama, Takashi; Arima, Yoshimi; Feng, Liping; Suzuki, Misao; Takeya, Motohiro; Saya, Hideyuki.

In: Oncogene, Vol. 23, No. 54, 18.11.2004, p. 8720-8730.

Research output: Contribution to journalArticle

Zhang, D, Hirota, T, Marumoto, T, Shimizu, M, Kunitoku, N, Sasayama, T, Arima, Y, Feng, L, Suzuki, M, Takeya, M & Saya, H 2004, 'Cre-loxP-controlled periodic Aurora-A overexpression induces mitotic abnormalities and hyperplasia in mammary glands of mouse models', Oncogene, vol. 23, no. 54, pp. 8720-8730. https://doi.org/10.1038/sj.onc.1208153
Zhang, Dongwei ; Hirota, Toru ; Marumoto, Tomotoshi ; Shimizu, Michio ; Kunitoku, Naoko ; Sasayama, Takashi ; Arima, Yoshimi ; Feng, Liping ; Suzuki, Misao ; Takeya, Motohiro ; Saya, Hideyuki. / Cre-loxP-controlled periodic Aurora-A overexpression induces mitotic abnormalities and hyperplasia in mammary glands of mouse models. In: Oncogene. 2004 ; Vol. 23, No. 54. pp. 8720-8730.
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