Creating a Saccharomyces cerevisiae haploid strain having 21 chromosomes

Donny Widianto, Eishi Yamamoto, Minetaka Sugiyama, Yukio Mukai, Yoshinobu Kaneko, Yasuji Oshima, Masafumi Nishizawa, Satoshi Harashima

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Chromosome engineering techniques that can manipulate a large segment of chromosomal DNA are useful not only for studying the organization of eukaryotic genomes but also for the improvement of industrially important strains. Toward the development of techniques that can efficiently manipulate a large segment of chromosome, we have previously reported a one-step chromosome splitting technique in a haploid Saccharomyces cerevisiae cell, with which we could successfully split yeast chromosome II, XIII, or XI into two halves to create a haploid strain having 17 chromosomes. We have now constructed chromosome splitting vectors bearing ADE2, HIS3, LEU2, or TRP1 marker, and by using these vectors, we could successively split yeast chromosomes to create a novel yeast haploid strain having up to 21 chromosomes. The specific growth rates of yeast strains carrying more than 16 chromosomes up to 21 did not differ significantly, suggesting that yeast cells can harbor more chromosomes than they do in their natural state, that is, 16 chromosomes, without serious effects on their growth.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume95
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1

Keywords

  • Chromosome splitting
  • Genome technology
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Widianto, D., Yamamoto, E., Sugiyama, M., Mukai, Y., Kaneko, Y., Oshima, Y., Nishizawa, M., & Harashima, S. (2003). Creating a Saccharomyces cerevisiae haploid strain having 21 chromosomes. Journal of Bioscience and Bioengineering, 95(1), 89-94. https://doi.org/10.1263/jbb.95.89