Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination

I. Oishi, K. Iwai, Y. Kagohashi, H. Fujimoto, K. I. Kariya, T. Kataoka, H. Sawa, Hideyuki Okano, H. Otani, H. Yamamura, Y. Minami

Research output: Contribution to journalArticle

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Abstract

Although chromosomal segregation at meiosis I is the critical process for genetic reassortment and inheritance, little is known about molecules involved in this process in metazoa. Here we show by utilizing double-stranded RNA (dsRNA)-mediated genetic interference that novel protein kinases (Ce-CDS-1 and Ce-CDS-2) related to Cds1 (Chk2) play an essential role in meiotic recombination in Caenorhabditis elegans. Injection of dsRNA into adult animals resulted in the inhibition of meiotic crossing over and induced the loss of chiasmata at diakenesis in oocytes of F1 animals. However, electron microscopic analysis revealed that synaptonemal complex formation in pachytene nuclei of the same progeny of injected animals appeared to be normal. Thus, Ce-CDS-1 and Ce-CDS-2 are the first example of Cds1-related kinases that are required for meiotic recombination in multicellular organisms.

Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalMolecular and Cellular Biology
Volume21
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Caenorhabditis elegans
Genetic Recombination
Phosphotransferases
Double-Stranded RNA
Genetic Phenomena
Synaptonemal Complex
Meiosis
Protein Kinases
Oocytes
Electrons
Injections

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Oishi, I., Iwai, K., Kagohashi, Y., Fujimoto, H., Kariya, K. I., Kataoka, T., ... Minami, Y. (2001). Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination. Molecular and Cellular Biology, 21(4), 1329-1335. https://doi.org/10.1128/MCB.21.4.1329-1335.2001

Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination. / Oishi, I.; Iwai, K.; Kagohashi, Y.; Fujimoto, H.; Kariya, K. I.; Kataoka, T.; Sawa, H.; Okano, Hideyuki; Otani, H.; Yamamura, H.; Minami, Y.

In: Molecular and Cellular Biology, Vol. 21, No. 4, 2001, p. 1329-1335.

Research output: Contribution to journalArticle

Oishi, I, Iwai, K, Kagohashi, Y, Fujimoto, H, Kariya, KI, Kataoka, T, Sawa, H, Okano, H, Otani, H, Yamamura, H & Minami, Y 2001, 'Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination', Molecular and Cellular Biology, vol. 21, no. 4, pp. 1329-1335. https://doi.org/10.1128/MCB.21.4.1329-1335.2001
Oishi, I. ; Iwai, K. ; Kagohashi, Y. ; Fujimoto, H. ; Kariya, K. I. ; Kataoka, T. ; Sawa, H. ; Okano, Hideyuki ; Otani, H. ; Yamamura, H. ; Minami, Y. / Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 4. pp. 1329-1335.
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