Critical role of dendritic cells in determining the Th 1/Th2 balance upon Leishmania major infection

Kazutomo Suzue, Seiki Kobayashi, Tsutomu Takeuchi, Mamoru Suzuki, Shigeo Koyasu

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The onset of Th1 immunity is in part regulated by genetic background. To elucidate the cell type carrying critical factors determining the Th1 response, we employed Rag-2-/- mice on Leishmania major-susceptible BALB/c and -resistant B10.D2 backgrounds. By using bone marrow (BM) chimeras generated by the transplantation of B10.D2 BM cells into BALB/c-Rag-2-/- mice, and vice versa, it was shown that hematopoietic cells carry factors determining the disease outcome and Th1 response against L. major infection. B10.D2-Rag-2-/- mice reconstituted with BALB/c CD4+ T cells exhibited a Th1 response and controlled L. major infection. Wild-type BALB/c mice inoculated with L. major-parasitized B10.D2-Rag-2-/- splenocytes also exhibited a Th1 response and a mild disease outcome, whereas such a Th1 response was not induced when CD11c+ dendritic cells (DCs) were depleted from parasitized B10.D2-Rag-2-/- splenocytes. Th1 response was reconstituted by the addition of L. major -parasitized B10.D2 DCs but not L. major-parasitized BALB/c DCs to DC-depleted parasitized B10.D2-Rag-2-/- splenocytes. These results indicate that DCs determine the outcome of the disease upon L. major infection.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalInternational immunology
Volume20
Issue number3
DOIs
Publication statusPublished - 2008 Mar

Keywords

  • Antigen-presenting cell
  • Infectious disease
  • Protozoan parasite
  • T

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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