Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis

Nobuyuki Takakura, Xu Ling Huang, Takeshi Naruse, Isao Hamaguchi, Daniel J. Dumont, George D. Yancopoulos, Toshio Suda

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Abstract

We have investigated the function of TIE2/TEK receptor tyrosine kinase in the development of definitive hematopoiesis. In the vitelline artery at 9.5 days post-coitum (d.p.c.), TIE2+ hematopoietic cells aggregated and adhered to TIE2+ endothelial cells. Soluble TIE2-Fc chimeric protein inhibited the development of hematopoiesis and angiogenesis in the para- aortic splanchnopleural mesoderm (P-Sp) explant culture, and TIE2-deficient mice showed severely impaired definitive hematopoiesis. An in vitro study revealed that Angiopoietin-1 but not Angiopoietin-2 promoted the adhesion to fibronectin (FN) through integrins in TIE2-transfected cells and primary TIE2+ cells sorted from 9.5 d.p.c. P-Sp. Adhesion of TIE2+ cells induced by Angiopoietin-1 enhanced the proliferation of hematopoietic progenitor cells.

Original languageEnglish
Pages (from-to)677-686
Number of pages10
JournalImmunity
Volume9
Issue number5
DOIs
Publication statusPublished - 1998 Nov

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Takakura, N., Huang, X. L., Naruse, T., Hamaguchi, I., Dumont, D. J., Yancopoulos, G. D., & Suda, T. (1998). Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis. Immunity, 9(5), 677-686. https://doi.org/10.1016/S1074-7613(00)80665-2