Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production In Crohn's disease

Shinta Mizuno, Yohei Mikami, Nobuhiko Kamada, Tango Handa, Atsushi Hayashi, Toshiro Sato, Katsuyoshi Matsuoka, Mami Matano, Yuki Ohta, Akira Sugita, Kazutaka Koganei, Rikisaburo Sahara, Masakazu Takazoe, Tadakazu Hisamatsu, Takanori Kanai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Interleukin (IL)-22-producing RORγt + innate lymphoid cells (ILCs) play a pivotal role in intestinal immunity. Recent reports demonstrated that ILCs contribute to mucosal protection and intestinal inflammation in mice. In humans, numbers of ROR γt+ ILCs are significantly increased in the intestine of patients with Crohn's disease (CD), suggesting that ILCs may be associated with intestinal inflammation in CD. However, the mechanism by which ILCs are regulated in the intestine of patients with CD is poorly understood. This study aimed to determine the activation mechanism of intestinal ILCs in patients with CD. Methods: CD45+ lineage marker-ILCs were isolated from intestinal lamina propria of patients with CD. ILCs were then subdivided into 4 distinct populations based on the expression of CD56 and CD127. Purified ILC subsets were cocultured with intestinal CD14+ macrophages, and IL-22 production was evaluated. Results: CD127+CD56- and CD127 +CD56+ ILC, but not CD127-CD56+ or CD127-CD56- ILC, subsets expressed RORγt and produced IL-22. IL-22 production by these ILC subsets was enhanced when ILCs were cocultured with intestinal macrophages. IL-23 or cell-to-cell contact was required for macrophage-mediated activation of ILCs. IL-22 production by ILCs was perturbed in inflamed mucosa compared with noninflamed mucosa. IL-22 induced the expression of Reg1α and Claudin-1 in human intestinal epithelial organoids. Conclusions: RORγt+ ILCs might enhance mucosal barrier function through the upregulation of Reg1 α through production of IL-22. Although CD14+ macrophages augment intestinal inflammation in patients with CD, macrophages also promote a negative feedback pathway through the activation of IL-22 production by RORγt+ ILCs.

Original languageEnglish
Pages (from-to)1426-1434
Number of pages9
JournalInflammatory bowel diseases
Volume20
Issue number8
DOIs
Publication statusPublished - 2014 Aug

Keywords

  • Crohn's disease
  • IL-22
  • Macrophages

ASJC Scopus subject areas

  • Immunology and Allergy
  • Gastroenterology

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    Mizuno, S., Mikami, Y., Kamada, N., Handa, T., Hayashi, A., Sato, T., Matsuoka, K., Matano, M., Ohta, Y., Sugita, A., Koganei, K., Sahara, R., Takazoe, M., Hisamatsu, T., & Kanai, T. (2014). Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production In Crohn's disease. Inflammatory bowel diseases, 20(8), 1426-1434. https://doi.org/10.1097/MIB.0000000000000105