Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment

Tomomi Konishi, Aya Mizutani Akimoto, Taihei Nishimoto, Yuki Tokura, Mizuki Tenjimbayashi, Kenta Homma, Ko Matsukawa, Taisei Kaku, Yuki Hiruta, Kenichi Nagase, Hideko Kanazawa, Seimei Shiratori

Research output: Contribution to journalArticle

Abstract

Stimuli-responsive smart materials are a key to the realization of next-generation medical technologies. Among them, the temperature-responsive polymer poly(N-isopropylacrylamide) (PNIPAAm) is attracting particular attention because it is easy to use in physiological conditions. PNIPAAm-grafted surfaces can undergo temperature-modulated cell adhesion and detachment without proteolytic enzymes, and can be used as cell-separating materials through selective cell adhesion/detachment. However, cell detachment at reduced temperatures is problematic because it takes several hours. A novel thermoresponsive crosslinked microfiber system that can greatly reduce the cell detachment time is introduced in this study. The crosslinked fibers provide temperature-dependent volume change, and enable cell detachment within 10 min of reducing the temperature, which is one-sixth of the time required in previous studies. The prompt cell detachment is thought to arise from a completely new mechanism derived from fiber swelling. This system will make a significant contribution as a novel cell manipulating system for next-generation medical technology.

Original languageEnglish
Article number1900464
JournalMacromolecular Rapid Communications
DOIs
Publication statusAccepted/In press - 2019 Jan 1

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Keywords

  • fast cell detachment
  • microfibers
  • thermoresponsive polymers
  • volume expansion

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Konishi, T., Mizutani Akimoto, A., Nishimoto, T., Tokura, Y., Tenjimbayashi, M., Homma, K., ... Shiratori, S. (Accepted/In press). Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment. Macromolecular Rapid Communications, [1900464]. https://doi.org/10.1002/marc.201900464