CrxOS maintains the self-renewal capacity of murine embryonic stem cells

Ryota Saito; Tokiwa Yamasaki; Yoko Nagai; Jinzhan Wu; Hiroaki Kajiho; Tadashi Yokoi; Eiichiro Noda; Sachiko Nishina; Hitoshi Niwa; Noriyuki Azuma; Toshiaki Katada; Hiroshi Nishina

doi:10.1016/j.bbrc.2009.09.118

CrxOS maintains the self-renewal capacity of murine embryonic stem cells

Ryota Saito, Tokiwa Yamasaki, Yoko Nagai, Jinzhan Wu, Hiroaki Kajiho, Tadashi Yokoi, Eiichiro Noda, Sachiko Nishina, Hitoshi Niwa, Noriyuki Azuma, Toshiaki Katada, Hiroshi Nishina

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Embryonic stem (ES) cells maintain pluripotency by self-renewal. Several homeoproteins, including Oct3/4 and Nanog, are known to be key factors in maintaining the self-renewal capacity of ES cells. However, other genes required for the mechanisms underlying this process are still unclear. Here we report the identification by in silico analysis of a homeobox-containing gene, CrxOS, that is specifically expressed in murine ES cells and is essential for their self-renewal. ES cells mainly express the short isoform of endogenous CrxOS. Using a polyoma-based episomal expression system, we demonstrate that overexpression of the CrxOS short isoform is sufficient for maintaining the undifferentiated morphology of ES cells and stimulating their proliferation. Finally, using RNA interference, we show that CrxOS is essential for the self-renewal of ES cells, and provisionally identify foxD3 as a downstream target gene of CrxOS. To our knowledge, ours is the first delineation of the physiological role of CrxOS in ES cells.

Original language	English
Pages (from-to)	1129-1135
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	390
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.09.118
Publication status	Published - 2009 Dec 25
Externally published	Yes

Keywords

CrxOS
Embryonic stem cell
Homeoprotein
In silico
Self-renewal
foxD3

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2009.09.118

Cite this

@article{5ca46bb28e8d42c8a70c0888254d0369,

title = "CrxOS maintains the self-renewal capacity of murine embryonic stem cells",

abstract = "Embryonic stem (ES) cells maintain pluripotency by self-renewal. Several homeoproteins, including Oct3/4 and Nanog, are known to be key factors in maintaining the self-renewal capacity of ES cells. However, other genes required for the mechanisms underlying this process are still unclear. Here we report the identification by in silico analysis of a homeobox-containing gene, CrxOS, that is specifically expressed in murine ES cells and is essential for their self-renewal. ES cells mainly express the short isoform of endogenous CrxOS. Using a polyoma-based episomal expression system, we demonstrate that overexpression of the CrxOS short isoform is sufficient for maintaining the undifferentiated morphology of ES cells and stimulating their proliferation. Finally, using RNA interference, we show that CrxOS is essential for the self-renewal of ES cells, and provisionally identify foxD3 as a downstream target gene of CrxOS. To our knowledge, ours is the first delineation of the physiological role of CrxOS in ES cells.",

keywords = "CrxOS, Embryonic stem cell, Homeoprotein, In silico, Self-renewal, foxD3",

author = "Ryota Saito and Tokiwa Yamasaki and Yoko Nagai and Jinzhan Wu and Hiroaki Kajiho and Tadashi Yokoi and Eiichiro Noda and Sachiko Nishina and Hitoshi Niwa and Noriyuki Azuma and Toshiaki Katada and Hiroshi Nishina",

note = "Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We thank Dr. Max Gassmann for MG1.19 ES cells and the pPyCAGIP episomal expression vector. We are grateful to numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions. ",

year = "2009",

month = dec,

day = "25",

doi = "10.1016/j.bbrc.2009.09.118",

language = "English",

volume = "390",

pages = "1129--1135",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - CrxOS maintains the self-renewal capacity of murine embryonic stem cells

AU - Saito, Ryota

AU - Yamasaki, Tokiwa

AU - Nagai, Yoko

AU - Wu, Jinzhan

AU - Kajiho, Hiroaki

AU - Yokoi, Tadashi

AU - Noda, Eiichiro

AU - Nishina, Sachiko

AU - Niwa, Hitoshi

AU - Azuma, Noriyuki

AU - Katada, Toshiaki

AU - Nishina, Hiroshi

N1 - Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We thank Dr. Max Gassmann for MG1.19 ES cells and the pPyCAGIP episomal expression vector. We are grateful to numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions.

PY - 2009/12/25

Y1 - 2009/12/25

N2 - Embryonic stem (ES) cells maintain pluripotency by self-renewal. Several homeoproteins, including Oct3/4 and Nanog, are known to be key factors in maintaining the self-renewal capacity of ES cells. However, other genes required for the mechanisms underlying this process are still unclear. Here we report the identification by in silico analysis of a homeobox-containing gene, CrxOS, that is specifically expressed in murine ES cells and is essential for their self-renewal. ES cells mainly express the short isoform of endogenous CrxOS. Using a polyoma-based episomal expression system, we demonstrate that overexpression of the CrxOS short isoform is sufficient for maintaining the undifferentiated morphology of ES cells and stimulating their proliferation. Finally, using RNA interference, we show that CrxOS is essential for the self-renewal of ES cells, and provisionally identify foxD3 as a downstream target gene of CrxOS. To our knowledge, ours is the first delineation of the physiological role of CrxOS in ES cells.

AB - Embryonic stem (ES) cells maintain pluripotency by self-renewal. Several homeoproteins, including Oct3/4 and Nanog, are known to be key factors in maintaining the self-renewal capacity of ES cells. However, other genes required for the mechanisms underlying this process are still unclear. Here we report the identification by in silico analysis of a homeobox-containing gene, CrxOS, that is specifically expressed in murine ES cells and is essential for their self-renewal. ES cells mainly express the short isoform of endogenous CrxOS. Using a polyoma-based episomal expression system, we demonstrate that overexpression of the CrxOS short isoform is sufficient for maintaining the undifferentiated morphology of ES cells and stimulating their proliferation. Finally, using RNA interference, we show that CrxOS is essential for the self-renewal of ES cells, and provisionally identify foxD3 as a downstream target gene of CrxOS. To our knowledge, ours is the first delineation of the physiological role of CrxOS in ES cells.

KW - CrxOS

KW - Embryonic stem cell

KW - Homeoprotein

KW - In silico

KW - Self-renewal

KW - foxD3

UR - http://www.scopus.com/inward/record.url?scp=70450270661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70450270661&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.09.118

DO - 10.1016/j.bbrc.2009.09.118

M3 - Article

C2 - 19800316

AN - SCOPUS:70450270661

SN - 0006-291X

VL - 390

SP - 1129

EP - 1135

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

CrxOS maintains the self-renewal capacity of murine embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this