Crystal structures of copper-depleted and copper-bound fungal pro-tyrosinase: Insights into endogenous cysteine-dependent copper incorporation

Nobutaka Fujieda, Shintaro Yabuta, Takuya Ikeda, Takuji Oyama, Norifumi Muraki, Genji Kurisu, Shinobu Itoh

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Background: Fungal tyrosinase maturation involves multiple processes of the dinuclear copper assembly and proteolytic activation. Results: Structural examinations and mutational studies of the pro-tyrosinases revealed that three endogenous cysteines contribute to the copper incorporation. Conclusion: The three highly flexible cysteines are essential for assembly of the active site across the protein shell. Significance: Elucidation of such a copper incorporation process provides useful insights into metal homeostasis.

Original languageEnglish
Pages (from-to)22128-22140
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number30
DOIs
Publication statusPublished - 2013 Jul 26
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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