Crystallization and preliminary X-ray studies of ferredoxin-NAD(P) + reductase from Chlorobium tepidum

Norifumi Muraki, Daisuke Seo, Tomoo Shiba, Takeshi Sakurai, Genji Kurisu

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Ferredoxin-NAD(P)+ reductase (FNR) is a key enzyme that catalyzes the photoreduction of NAD(P)+ to generate NAD(P)H during the final step of the photosynthetic electron-transport chain. FNR from the green sulfur bacterium Chlorobium tepidum is a homodimeric enzyme with a molecular weight of 90 kDa; it shares a high level of amino-acid sequence identity to thioredoxin reductase rather than to conventional plant-type FNRs. In order to understand the structural basis of the ferredoxin-dependency of this unique photosynthetic FNR, C. tepidum FNR has been heterologously expressed, purified and crystallized in two forms. Form I crystals belong to space group C2221 and contain one dimer in the asymmetric unit, while form II crystals belong to space group P4122 or P4322. Diffraction data were collected from a form I crystal to 2.4 Å resolution on the synchrotron-radiation beamline NW12 at the Photon Factory.

Original languageEnglish
Pages (from-to)186-189
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number3
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Ferredoxin-NAD(P) reductase
  • Photosynthesis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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