Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120

Rei Narikawa, Norifumi Muraki, Tomoo Shiba, Masahiko Ikeuchi, Genji Kurisu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cyanobacteriochromes form a recently defined superfamily of tetrapyrrole-based photoreceptors that are distantly related to conventional red/far-red photoreceptor phytochromes. Among these molecules, AnPixJ from Anabaena sp. PCC 7120 is a novel photoreceptor that shows reversible photoconversion between green-absorbing and red-absorbing forms, which is in contrast to the properties of conventional phytochromes. In order to better understand the structural basis of this unique photoconversion mechanism, the chromophore-binding domain of AnPixJ (AnPixJ-GAF2) was heterologously overproduced and purified, and crystallization of both forms was attempted. Blue crystals of the red-absorbing form of AnPixJ-GAF2 were successfully obtained; they belonged to space group P43212 and contained one monomer per asymmetric unit. Diffraction data were collected to a resolution of 1.8 Å using synchrotron-radiation beamline BL-5A at the Photon Factory.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • AnPixJ
  • Chromophore-binding domains
  • Cyanobacteriochromes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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