Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line

Yumiko Matsubara, Mitsuru Murata, Yasuo Ikeda

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

The molecular mechanisms whereby stem cells develop into platelet-producing megakaryocytes (MKs) are not yet fully understood. Within this chapter we describe a two-step in vitro culture system in which MKs and platelets are generated from primary subcutaneous adipose tissues and the preadipocyte cell line 3T3L1. The cells are first cultured in an adipocyte induction medium for 10-12 days, followed by 8-14 days culture in a MK differentiation medium. Adipose tissue-derived MKs and platelets display a number of morphological and functional characteristics (e.g., secretory granules, open canalicular membranes) comparable with the native cell type. The use of subcutaneous adipose tissue to produce a large number of platelets is advantageous because this tissue is easily obtained and available in large quantities. Thus, this in vitro culture system may prove useful in both regenerative medicine, but it may also be used in understanding fundamental research questions within MK and platelet research, including further elucidation of the pathways that cause cells to differentiate along the MK lineage ultimately leading to platelet production.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages249-258
Number of pages10
Volume788
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume788
ISSN (Print)10643745

Fingerprint

Megakaryocytes
Subcutaneous Fat
Blood Platelets
Cell Line
Regenerative Medicine
Secretory Vesicles
Platelet Count
Research
Adipocytes
Adipose Tissue
Cultured Cells
Stem Cells
Membranes

Keywords

  • Adipose tissue
  • Megakaryopoiesis
  • Preadipocyte
  • Stem cell
  • Thrombopoiesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Matsubara, Y., Murata, M., & Ikeda, Y. (2012). Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. In Methods in Molecular Biology (Vol. 788, pp. 249-258). (Methods in Molecular Biology; Vol. 788). https://doi.org/10.1007/978-1-61779-307-3_17

Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. / Matsubara, Yumiko; Murata, Mitsuru; Ikeda, Yasuo.

Methods in Molecular Biology. Vol. 788 2012. p. 249-258 (Methods in Molecular Biology; Vol. 788).

Research output: Chapter in Book/Report/Conference proceedingChapter

Matsubara, Y, Murata, M & Ikeda, Y 2012, Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. in Methods in Molecular Biology. vol. 788, Methods in Molecular Biology, vol. 788, pp. 249-258. https://doi.org/10.1007/978-1-61779-307-3_17
Matsubara Y, Murata M, Ikeda Y. Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. In Methods in Molecular Biology. Vol. 788. 2012. p. 249-258. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-307-3_17
Matsubara, Yumiko ; Murata, Mitsuru ; Ikeda, Yasuo. / Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. Methods in Molecular Biology. Vol. 788 2012. pp. 249-258 (Methods in Molecular Biology).
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