Culturing intestinal stem cells: Applications for colorectal cancer research

Masayuki Fujii, Toshiro Sato

Research output: Contribution to journalShort survey

4 Citations (Scopus)

Abstract

Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of intestinal stem cell self-renewal, leading to the development of novel human intestinal organoid culture system. Intestinal organoid culture enabled to expand normal or tumor epithelial cells in vitro retaining their stem cell self-renewal and multiple differentiation. Gene manipulation of these cultured cells may provide an attractive tool for investigating genetic events involved in colorectal carcinogenesis.

Original languageEnglish
Article number169
JournalFrontiers in Genetics
Volume5
Issue numberJUN
DOIs
Publication statusPublished - 2014 Jan 1

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Keywords

  • Cancer stem cells (CSC)
  • Niche
  • Organoids
  • R-spondin
  • Wnt proteins

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Genetics(clinical)

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