Curcumin and genistein additively potentiate G551D-CFTR

Ying Chun Yu, Haruna Miki, Yumi Nakamura, Akiko Hanyuda, Yohei Matsuzaki, Yoichiro Abe, Masato Yasui, Kazuhiko Tanaka, Tzyh Chang Hwang, Silvia G. Bompadre, Yoshiro Sohma

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: The G551D mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) is a common cause of cystic fibrosis (CF). G551D-CFTR is characterized by an extremely low open probability despite its normal trafficking to the plasma membrane. Numerous small molecules have been shown to increase the activity of G551D-CFTR presumably by binding to the CFTR protein. Methods: We investigated the effect of curcumin, genistein and their combined application on G551D-CFTR activity using the patch clamp technique. Results: Curcumin increased G551D-CFTR whole-cell and single-channel currents less than genistein did at their maximally effective concentrations. However, curcumin further increased the channel activity of G551D-CFTR that had been already maximally potentiated by genistein, up to ~. 50% of the WT-CFTR level. In addition, the combined application of genistein and curcumin over a lower concentration range synergistically rescued the gating defect of G551D-CFTR. Conclusions: The additive effects between curcumin and genistein not only support the hypothesis that multiple mechanisms are involved in the action of CFTR potentiators, but also pose pharmaceutical implications in the development of drugs for CF pharmacotherapy.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalJournal of Cystic Fibrosis
Volume10
Issue number4
DOIs
Publication statusPublished - 2011 Jul

Fingerprint

Cystic Fibrosis Transmembrane Conductance Regulator
Curcumin
Genistein
Cystic Fibrosis
Patch-Clamp Techniques
Pharmaceutical Preparations
Cell Membrane
Drug Therapy

Keywords

  • Additive effect
  • CFTR
  • Curcumin
  • Cystic fibrosis
  • G551D mutation
  • Genistein

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pediatrics, Perinatology, and Child Health

Cite this

Yu, Y. C., Miki, H., Nakamura, Y., Hanyuda, A., Matsuzaki, Y., Abe, Y., ... Sohma, Y. (2011). Curcumin and genistein additively potentiate G551D-CFTR. Journal of Cystic Fibrosis, 10(4), 243-252. https://doi.org/10.1016/j.jcf.2011.03.001

Curcumin and genistein additively potentiate G551D-CFTR. / Yu, Ying Chun; Miki, Haruna; Nakamura, Yumi; Hanyuda, Akiko; Matsuzaki, Yohei; Abe, Yoichiro; Yasui, Masato; Tanaka, Kazuhiko; Hwang, Tzyh Chang; Bompadre, Silvia G.; Sohma, Yoshiro.

In: Journal of Cystic Fibrosis, Vol. 10, No. 4, 07.2011, p. 243-252.

Research output: Contribution to journalArticle

Yu, YC, Miki, H, Nakamura, Y, Hanyuda, A, Matsuzaki, Y, Abe, Y, Yasui, M, Tanaka, K, Hwang, TC, Bompadre, SG & Sohma, Y 2011, 'Curcumin and genistein additively potentiate G551D-CFTR', Journal of Cystic Fibrosis, vol. 10, no. 4, pp. 243-252. https://doi.org/10.1016/j.jcf.2011.03.001
Yu, Ying Chun ; Miki, Haruna ; Nakamura, Yumi ; Hanyuda, Akiko ; Matsuzaki, Yohei ; Abe, Yoichiro ; Yasui, Masato ; Tanaka, Kazuhiko ; Hwang, Tzyh Chang ; Bompadre, Silvia G. ; Sohma, Yoshiro. / Curcumin and genistein additively potentiate G551D-CFTR. In: Journal of Cystic Fibrosis. 2011 ; Vol. 10, No. 4. pp. 243-252.
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