Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet

Kohei Tsuchiya, Taisuke Mori, Guoping Chen, Takashi Ushida, Tetsuya Tateishi, Takeo Matsuno, Michiie Sakamoto, Akihiro Umezawa

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

New bone for the repair or the restoration of the function of traumatized, damaged, or lost bone is a major clinical need, and bone tissue engineering has been heralded as an alternative strategy for regenerating bone. A novel web-like structured biodegradable hybrid sheet has been developed for bone tissue engineering by preparing knitted poly(DL-lactic-co-glycolic acid) sheets (PLGA sheets) with collagen microsponges in their openings. The PLGA skeleton facilitates the formation of the hybrid sheets into desired shapes, and the collagen microsponges in the pores of the PLGA sheet promote cell adhesion and uniform cell distribution throughout the sheet. A large number of osteoblasts established from marrow stroma adhere to the scaffolds and generate the desired-shaped bone in combination with these novel sheets. These results indicate that the web-like structured novel sheet shows promise for use as a tool for custom-shaped bone regeneration in basic research on osteogenesis and for the development of therapeutic applications.

Original languageEnglish
Pages (from-to)141-153
Number of pages13
JournalCell and Tissue Research
Volume316
Issue number2
DOIs
Publication statusPublished - 2004 May

Keywords

  • Bone regeneration
  • KUSA-A1 cells
  • Marrow stroma
  • Polymer
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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