Cutting edge: Role of IL-27/WSX-1 signaling for induction of T-bet through activation of STAT1 during initial Th1 commitment

Atsunobu Takeda, Shinjiro Hamano, Atsushi Yamanaka, Toshikatsu Hanada, Tatsuro Ishibashi, Tak W. Mak, Akihiko Yoshimura, Hiroki Yoshida

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419 Citations (Scopus)

Abstract

WSX-1 is a member of the class I cytokine receptor family with homology to IL-12Rβ2 and is essential for the initial mounting of Th1 responses. STAT1 interacts with tyrosine-phosphorylated WSX-1, and the conserved tyrosine residue of the cytoplasmic domain of WSX-1 is essential for transcriptional activation of STAT1. IL-27 stimulation induced STAT1 phosphorylation in wild-type but not in WSX-1-deficient naive CD4+ T cells. Although IL-27 did not directly induce IFN-γ production by wild-type CD4+ T cells, IL-12-dependent IFN-γ production was augmented by IL-27 stimulation in wild-type naive CD4+ T cells but was impaired in WSX-1-deficient naive CD4+ T cells. Additionally, IL-27 stimulation induced T-bet and IL-12Rβ2 expression in wild-type, but not in WSX-1-deficient, CD4+ T cells. Thus, during the initiation of Th1 differentiation, the IL-27/WSX-1 signaling system plays a pivotal role by STAT1-mediated T-bet induction before the IL-12R system.

Original languageEnglish
Pages (from-to)4886-4890
Number of pages5
JournalJournal of Immunology
Volume170
Issue number10
DOIs
Publication statusPublished - 2003 May 15
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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