CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice

Tokushige Oyama, Soichiro Miura, Chikako Watanabe, Ryota Hokari, Yoichi Fujiyama, Shunsuke Komoto, Yoshikazu Tsuzuki, Naoki Hosoe, Hiroshi Nagata, Toshifumi Hibi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective: Although it is known that the chemokines CXCL12 and CCL20 are expressed in the intestine, their contribution to lymphocyte homing has not been investigated in detail. The authors investigated whether the CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. Methods: Labeled lamina proprial lymphocytes (LPLs) were administered to mice, and their adhesion to microvessels of normal and TNF-α-induced inflamed intestinal mucosa was observed under an intravital microscope. Antibodies against CXCL12, CCL-20, or CCL-25 were administered prior to lymphocyte administration, and in some experiments CXCR4 or CCR6 on LPLs was desensitized with an excess amount of chemokine. Results: LPLs adhered to microvessels of the ileum and colon, and TNF-α induced a significant accumulation at both sites. Blocking of the CXCL12-CXCR4 system significantly inhibited the LPL adhesion in the ileum and colon under both normal and TNF-α-treated conditions. However, blocking of the CCL20-CCR6 system significantly attenuated LPL adhesion only under a TNF-α-treated condition. There was an additive inhibitory effect on LPL adherence by CXCL12 and CCL20 blocking in TNF-α-induced inflamed intestines. There was also an additive function of the CCL25-CCR9 system in LPL accumulation in the small intestine. Conclusion: Several chemokine systems may play significant roles cooperatively in vivo in LPL adherence to microvessels of intestinal mucosa.

Original languageEnglish
Pages (from-to)753-766
Number of pages14
JournalMicrocirculation
Volume14
Issue number7
DOIs
Publication statusPublished - 2007 Sep

Fingerprint

Microvessels
Lymphocytes
T-Lymphocytes
Intestinal Mucosa
Ileum
Chemokines
Small Intestine
Intestines
Chemokine CCL20
Colon
Chemokine CXCL12
Large Intestine
Antibodies

Keywords

  • CCL20-CCR6 system
  • CCL25-CCR9 system
  • CXCL12-CXCR4 system
  • Intestinal microvessels
  • Mucosal (lamina proprial) T lymphocytes
  • TNF-α,

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Molecular Biology

Cite this

Oyama, T., Miura, S., Watanabe, C., Hokari, R., Fujiyama, Y., Komoto, S., ... Hibi, T. (2007). CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice. Microcirculation, 14(7), 753-766. https://doi.org/10.1080/10739680701409993

CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice. / Oyama, Tokushige; Miura, Soichiro; Watanabe, Chikako; Hokari, Ryota; Fujiyama, Yoichi; Komoto, Shunsuke; Tsuzuki, Yoshikazu; Hosoe, Naoki; Nagata, Hiroshi; Hibi, Toshifumi.

In: Microcirculation, Vol. 14, No. 7, 09.2007, p. 753-766.

Research output: Contribution to journalArticle

Oyama, T, Miura, S, Watanabe, C, Hokari, R, Fujiyama, Y, Komoto, S, Tsuzuki, Y, Hosoe, N, Nagata, H & Hibi, T 2007, 'CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice', Microcirculation, vol. 14, no. 7, pp. 753-766. https://doi.org/10.1080/10739680701409993
Oyama, Tokushige ; Miura, Soichiro ; Watanabe, Chikako ; Hokari, Ryota ; Fujiyama, Yoichi ; Komoto, Shunsuke ; Tsuzuki, Yoshikazu ; Hosoe, Naoki ; Nagata, Hiroshi ; Hibi, Toshifumi. / CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice. In: Microcirculation. 2007 ; Vol. 14, No. 7. pp. 753-766.
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abstract = "Objective: Although it is known that the chemokines CXCL12 and CCL20 are expressed in the intestine, their contribution to lymphocyte homing has not been investigated in detail. The authors investigated whether the CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. Methods: Labeled lamina proprial lymphocytes (LPLs) were administered to mice, and their adhesion to microvessels of normal and TNF-α-induced inflamed intestinal mucosa was observed under an intravital microscope. Antibodies against CXCL12, CCL-20, or CCL-25 were administered prior to lymphocyte administration, and in some experiments CXCR4 or CCR6 on LPLs was desensitized with an excess amount of chemokine. Results: LPLs adhered to microvessels of the ileum and colon, and TNF-α induced a significant accumulation at both sites. Blocking of the CXCL12-CXCR4 system significantly inhibited the LPL adhesion in the ileum and colon under both normal and TNF-α-treated conditions. However, blocking of the CCL20-CCR6 system significantly attenuated LPL adhesion only under a TNF-α-treated condition. There was an additive inhibitory effect on LPL adherence by CXCL12 and CCL20 blocking in TNF-α-induced inflamed intestines. There was also an additive function of the CCL25-CCR9 system in LPL accumulation in the small intestine. Conclusion: Several chemokine systems may play significant roles cooperatively in vivo in LPL adherence to microvessels of intestinal mucosa.",
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T1 - CXCL 12 and CCL20 play a significant role in mucosal T-lymphocyte adherence to intestinal microvessels in mice

AU - Oyama, Tokushige

AU - Miura, Soichiro

AU - Watanabe, Chikako

AU - Hokari, Ryota

AU - Fujiyama, Yoichi

AU - Komoto, Shunsuke

AU - Tsuzuki, Yoshikazu

AU - Hosoe, Naoki

AU - Nagata, Hiroshi

AU - Hibi, Toshifumi

PY - 2007/9

Y1 - 2007/9

N2 - Objective: Although it is known that the chemokines CXCL12 and CCL20 are expressed in the intestine, their contribution to lymphocyte homing has not been investigated in detail. The authors investigated whether the CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. Methods: Labeled lamina proprial lymphocytes (LPLs) were administered to mice, and their adhesion to microvessels of normal and TNF-α-induced inflamed intestinal mucosa was observed under an intravital microscope. Antibodies against CXCL12, CCL-20, or CCL-25 were administered prior to lymphocyte administration, and in some experiments CXCR4 or CCR6 on LPLs was desensitized with an excess amount of chemokine. Results: LPLs adhered to microvessels of the ileum and colon, and TNF-α induced a significant accumulation at both sites. Blocking of the CXCL12-CXCR4 system significantly inhibited the LPL adhesion in the ileum and colon under both normal and TNF-α-treated conditions. However, blocking of the CCL20-CCR6 system significantly attenuated LPL adhesion only under a TNF-α-treated condition. There was an additive inhibitory effect on LPL adherence by CXCL12 and CCL20 blocking in TNF-α-induced inflamed intestines. There was also an additive function of the CCL25-CCR9 system in LPL accumulation in the small intestine. Conclusion: Several chemokine systems may play significant roles cooperatively in vivo in LPL adherence to microvessels of intestinal mucosa.

AB - Objective: Although it is known that the chemokines CXCL12 and CCL20 are expressed in the intestine, their contribution to lymphocyte homing has not been investigated in detail. The authors investigated whether the CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. Methods: Labeled lamina proprial lymphocytes (LPLs) were administered to mice, and their adhesion to microvessels of normal and TNF-α-induced inflamed intestinal mucosa was observed under an intravital microscope. Antibodies against CXCL12, CCL-20, or CCL-25 were administered prior to lymphocyte administration, and in some experiments CXCR4 or CCR6 on LPLs was desensitized with an excess amount of chemokine. Results: LPLs adhered to microvessels of the ileum and colon, and TNF-α induced a significant accumulation at both sites. Blocking of the CXCL12-CXCR4 system significantly inhibited the LPL adhesion in the ileum and colon under both normal and TNF-α-treated conditions. However, blocking of the CCL20-CCR6 system significantly attenuated LPL adhesion only under a TNF-α-treated condition. There was an additive inhibitory effect on LPL adherence by CXCL12 and CCL20 blocking in TNF-α-induced inflamed intestines. There was also an additive function of the CCL25-CCR9 system in LPL accumulation in the small intestine. Conclusion: Several chemokine systems may play significant roles cooperatively in vivo in LPL adherence to microvessels of intestinal mucosa.

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KW - Mucosal (lamina proprial) T lymphocytes

KW - TNF-α,

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