CXCR4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin-, and neuropeptide y-expressing gabaergic interneurons

Daisuke H. Tanaka, Sakae Mikami, Takashi Nagasawa, Jun Ichi Miyazaki, Kazunori Nakajima, Fujio Murakami

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cortical GABAergic interneurons are divided into various subtypes, with each subtype contributing to rich variety and fine details of inhibition. Despite the functional importance of each interneuron subtype, the molecular mechanisms that contribute to sorting them to their appropriate positions within the cortex remain unclear. Here, we show that the chemokine receptor CXCR4 regulates the regional and layer-specific distribution of interneuron subtypes. We removed Cxcr4 specifically in a subset of interneurons at a specific mouse embryonic developmental stage and analyzed the number of interneurons and their laminar distribution in 9 representative cortical regions comprehensively in adults. We found that the number of Cxcr4-deleted calretinin-and that of neuropeptide Y-expressing interneurons were reduced in most caudomedial and lateral cortical regions, respectively, and also in superficial layers. In addition, Cxcr4-deleted somatostatin-expressing interneurons showed a reduction in the number of superficial layers in certain cortical regions but of deep layers in others. These findings suggest that CXCR4 is required for proper regional and laminar distribution in a wider interneuron subpopulation than previously thought and may regulate the establishment of functional cortical circuitry in certain cortical regions and layers.

Original languageEnglish
Pages (from-to)2810-2817
Number of pages8
JournalCerebral Cortex
Volume20
Issue number12
DOIs
Publication statusPublished - 2010 Dec

Fingerprint

Calbindin 2
Interneurons
Somatostatin
Neuropeptides
Chemokine Receptors
Neuropeptide Y

Keywords

  • genetic fate mapping
  • inducible gene deletion
  • interneuron distribution
  • interneuron subtypes
  • temporally specific loss of function

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

CXCR4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin-, and neuropeptide y-expressing gabaergic interneurons. / Tanaka, Daisuke H.; Mikami, Sakae; Nagasawa, Takashi; Miyazaki, Jun Ichi; Nakajima, Kazunori; Murakami, Fujio.

In: Cerebral Cortex, Vol. 20, No. 12, 12.2010, p. 2810-2817.

Research output: Contribution to journalArticle

Tanaka, Daisuke H. ; Mikami, Sakae ; Nagasawa, Takashi ; Miyazaki, Jun Ichi ; Nakajima, Kazunori ; Murakami, Fujio. / CXCR4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin-, and neuropeptide y-expressing gabaergic interneurons. In: Cerebral Cortex. 2010 ; Vol. 20, No. 12. pp. 2810-2817.
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