Cyclic mechanical stretching and interleukin-1α synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells

D. Korita, Hiroshi Itoh, N. Sagawa, S. Yura, M. Yoshida, K. Kakui, M. Takemura, M. A. Nuamah, S. Fujii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Prostacyclin (PGI2), a potent uterine smooth muscle relaxant, is postulated to be a major prostaglandin (PG) secreted from the human myometrium. PGI2 metabolite concentrations in the maternal plasma were reported to be elevated during pregnancy, especially during labor. Recently, we developed cultured human myometrial cells from pregnant women and reported that cyclic mechanical stretching mimicking labor increased PGI 2 secretion from these cells by up-regulating PGI2 synthase promoter activities. Since elevation of cervical/vaginal interleukin-1α (IL-1α) concentrations is also a characteristic feature of delivery, and IL-1α is a known stimulator of PG synthesis, we investigated a possible synergistic effect of cyclic mechanical stretching and IL-1α on PGI2 production in cultured human myometrial cells. Treatment with IL-1α (10 ng/ml) significantly augmented (4- to 60-fold) the secretion of PGI2, prostaglandin E2 (PGE2), prostaglandin F (PGF) and thromboxane A2 (TXA2) from cultured human myometrial cells obtained from non-pregnant and pregnant women as well as in cultured human umbilical artery and cultured human coronary artery smooth muscle cells (p < 0.05 for all comparisons). However, labor-like cyclic mechanical stretching up-regulated IL-1α-augmented PGI2 secretion from myometrial cells obtained from non-pregnant and pregnant women 2.1- to 2.8-fold (p < 0.05 for all comparisons), but not PGE2, PGF nor TXA 2. Moreover, such an augumentation of PGI2 secretion by cyclic mechanical stretching was not observed in cultured human umbilical artery nor in cultured human coronary artery smooth muscle cells. These results suggest that cyclic mechanical stretching by labor, in concert with IL-1α stimulation, contributes to the increase in myometrial PGI2 secretion during delivery.

Original languageEnglish
Pages (from-to)130-137
Number of pages8
JournalGynecological Endocrinology
Volume18
Issue number3
DOIs
Publication statusPublished - 2004 Mar
Externally publishedYes

Fingerprint

Epoprostenol
Interleukin-1
Up-Regulation
Pregnant Women
Umbilical Arteries
Dinoprost
Myometrium
Dinoprostone
Prostaglandins
Smooth Muscle Myocytes
Coronary Vessels
Thromboxane A2
Interleukin-10
Smooth Muscle
Mothers
Pregnancy

Keywords

  • Interleukin-1α
  • Labor
  • Mechanical stretching
  • Parturition
  • Prostacyclin

ASJC Scopus subject areas

  • Endocrinology
  • Obstetrics and Gynaecology

Cite this

Cyclic mechanical stretching and interleukin-1α synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells. / Korita, D.; Itoh, Hiroshi; Sagawa, N.; Yura, S.; Yoshida, M.; Kakui, K.; Takemura, M.; Nuamah, M. A.; Fujii, S.

In: Gynecological Endocrinology, Vol. 18, No. 3, 03.2004, p. 130-137.

Research output: Contribution to journalArticle

Korita, D. ; Itoh, Hiroshi ; Sagawa, N. ; Yura, S. ; Yoshida, M. ; Kakui, K. ; Takemura, M. ; Nuamah, M. A. ; Fujii, S. / Cyclic mechanical stretching and interleukin-1α synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells. In: Gynecological Endocrinology. 2004 ; Vol. 18, No. 3. pp. 130-137.
@article{34ca2af991894c51a7a6885a41c7c4c9,
title = "Cyclic mechanical stretching and interleukin-1α synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells",
abstract = "Prostacyclin (PGI2), a potent uterine smooth muscle relaxant, is postulated to be a major prostaglandin (PG) secreted from the human myometrium. PGI2 metabolite concentrations in the maternal plasma were reported to be elevated during pregnancy, especially during labor. Recently, we developed cultured human myometrial cells from pregnant women and reported that cyclic mechanical stretching mimicking labor increased PGI 2 secretion from these cells by up-regulating PGI2 synthase promoter activities. Since elevation of cervical/vaginal interleukin-1α (IL-1α) concentrations is also a characteristic feature of delivery, and IL-1α is a known stimulator of PG synthesis, we investigated a possible synergistic effect of cyclic mechanical stretching and IL-1α on PGI2 production in cultured human myometrial cells. Treatment with IL-1α (10 ng/ml) significantly augmented (4- to 60-fold) the secretion of PGI2, prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and thromboxane A2 (TXA2) from cultured human myometrial cells obtained from non-pregnant and pregnant women as well as in cultured human umbilical artery and cultured human coronary artery smooth muscle cells (p < 0.05 for all comparisons). However, labor-like cyclic mechanical stretching up-regulated IL-1α-augmented PGI2 secretion from myometrial cells obtained from non-pregnant and pregnant women 2.1- to 2.8-fold (p < 0.05 for all comparisons), but not PGE2, PGF2α nor TXA 2. Moreover, such an augumentation of PGI2 secretion by cyclic mechanical stretching was not observed in cultured human umbilical artery nor in cultured human coronary artery smooth muscle cells. These results suggest that cyclic mechanical stretching by labor, in concert with IL-1α stimulation, contributes to the increase in myometrial PGI2 secretion during delivery.",
keywords = "Interleukin-1α, Labor, Mechanical stretching, Parturition, Prostacyclin",
author = "D. Korita and Hiroshi Itoh and N. Sagawa and S. Yura and M. Yoshida and K. Kakui and M. Takemura and Nuamah, {M. A.} and S. Fujii",
year = "2004",
month = "3",
doi = "10.1080/09513590410001667850",
language = "English",
volume = "18",
pages = "130--137",
journal = "Gynecological Endocrinology",
issn = "0951-3590",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Cyclic mechanical stretching and interleukin-1α synergistically up-regulate prostacyclin secretion in cultured human uterine myometrial cells

AU - Korita, D.

AU - Itoh, Hiroshi

AU - Sagawa, N.

AU - Yura, S.

AU - Yoshida, M.

AU - Kakui, K.

AU - Takemura, M.

AU - Nuamah, M. A.

AU - Fujii, S.

PY - 2004/3

Y1 - 2004/3

N2 - Prostacyclin (PGI2), a potent uterine smooth muscle relaxant, is postulated to be a major prostaglandin (PG) secreted from the human myometrium. PGI2 metabolite concentrations in the maternal plasma were reported to be elevated during pregnancy, especially during labor. Recently, we developed cultured human myometrial cells from pregnant women and reported that cyclic mechanical stretching mimicking labor increased PGI 2 secretion from these cells by up-regulating PGI2 synthase promoter activities. Since elevation of cervical/vaginal interleukin-1α (IL-1α) concentrations is also a characteristic feature of delivery, and IL-1α is a known stimulator of PG synthesis, we investigated a possible synergistic effect of cyclic mechanical stretching and IL-1α on PGI2 production in cultured human myometrial cells. Treatment with IL-1α (10 ng/ml) significantly augmented (4- to 60-fold) the secretion of PGI2, prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and thromboxane A2 (TXA2) from cultured human myometrial cells obtained from non-pregnant and pregnant women as well as in cultured human umbilical artery and cultured human coronary artery smooth muscle cells (p < 0.05 for all comparisons). However, labor-like cyclic mechanical stretching up-regulated IL-1α-augmented PGI2 secretion from myometrial cells obtained from non-pregnant and pregnant women 2.1- to 2.8-fold (p < 0.05 for all comparisons), but not PGE2, PGF2α nor TXA 2. Moreover, such an augumentation of PGI2 secretion by cyclic mechanical stretching was not observed in cultured human umbilical artery nor in cultured human coronary artery smooth muscle cells. These results suggest that cyclic mechanical stretching by labor, in concert with IL-1α stimulation, contributes to the increase in myometrial PGI2 secretion during delivery.

AB - Prostacyclin (PGI2), a potent uterine smooth muscle relaxant, is postulated to be a major prostaglandin (PG) secreted from the human myometrium. PGI2 metabolite concentrations in the maternal plasma were reported to be elevated during pregnancy, especially during labor. Recently, we developed cultured human myometrial cells from pregnant women and reported that cyclic mechanical stretching mimicking labor increased PGI 2 secretion from these cells by up-regulating PGI2 synthase promoter activities. Since elevation of cervical/vaginal interleukin-1α (IL-1α) concentrations is also a characteristic feature of delivery, and IL-1α is a known stimulator of PG synthesis, we investigated a possible synergistic effect of cyclic mechanical stretching and IL-1α on PGI2 production in cultured human myometrial cells. Treatment with IL-1α (10 ng/ml) significantly augmented (4- to 60-fold) the secretion of PGI2, prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and thromboxane A2 (TXA2) from cultured human myometrial cells obtained from non-pregnant and pregnant women as well as in cultured human umbilical artery and cultured human coronary artery smooth muscle cells (p < 0.05 for all comparisons). However, labor-like cyclic mechanical stretching up-regulated IL-1α-augmented PGI2 secretion from myometrial cells obtained from non-pregnant and pregnant women 2.1- to 2.8-fold (p < 0.05 for all comparisons), but not PGE2, PGF2α nor TXA 2. Moreover, such an augumentation of PGI2 secretion by cyclic mechanical stretching was not observed in cultured human umbilical artery nor in cultured human coronary artery smooth muscle cells. These results suggest that cyclic mechanical stretching by labor, in concert with IL-1α stimulation, contributes to the increase in myometrial PGI2 secretion during delivery.

KW - Interleukin-1α

KW - Labor

KW - Mechanical stretching

KW - Parturition

KW - Prostacyclin

UR - http://www.scopus.com/inward/record.url?scp=1542297634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542297634&partnerID=8YFLogxK

U2 - 10.1080/09513590410001667850

DO - 10.1080/09513590410001667850

M3 - Article

C2 - 15255281

AN - SCOPUS:1542297634

VL - 18

SP - 130

EP - 137

JO - Gynecological Endocrinology

JF - Gynecological Endocrinology

SN - 0951-3590

IS - 3

ER -