Cyclic strain induces reactive oxygen species production via an endothelial NAD(P)H oxidase

Hidetsugu Saito, Shinichiro Tada, Hirotoshi Ebinuma, Kanji Wakabayashi, Tamako Takagi, Yoshimasa Saito, Nobuhiro Nakamoto, Satoshi Kurita, Hiromasa Ishii

Research output: Contribution to journalArticle

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Abstract

Vascular endothelial cells are constantly subjected to pressure-induced cyclic strain. Reactive oxygen species (ROS) have been implicated in atherosclerosis and vascular remodeling. Recent evidence indicates that a vascular NAD(P)H oxidase may be an important source of ROS in both physiologic and pathophysiologic situations. The aim of this study was to investigate cyclic strain-induced NAD(P)H oxidase activity in endothelial cells. ROS production was examined by electron paramagnetic resonance and lucigenin chemiluminescence. Cyclic strain-induced NAD(P)H oxidase activity was quantified by activity assay while the expression of p22phox was monitored by Northern blotting. Endothelial cells produce basal amounts of ROS that were enhanced by cyclic strain. Moreover subsequent stimulation with TNF-α resulted in significantly greater ROS production in cells previously exposed to cyclic strain as compared to static conditions. Cyclic strain resulted in a significant increase in message for the p22phox subunit as well as activity of the NAD(P)H oxidase. The induced oxidative stress was accompanied by increased mobilization of the transcription factor N FκB, an effect that was blocked by a pharmacological inhibitor of NAD(P)H. These results demonstrate a pivotal role for NAD(P)H oxidase in cyclic strain-induced endothelial ROS production and may provide insight into the modulation of vascular disease by biomechanical forces.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalJournal of Cellular Biochemistry
Volume81
Issue numberSUPPL. 36
DOIs
Publication statusPublished - 2001

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NADPH Oxidase
Reactive Oxygen Species
Endothelial cells
Endothelial Cells
Electron Spin Resonance Spectroscopy
Oxidative stress
Luminescence
Chemiluminescence
Vascular Diseases
Northern Blotting
NAD
Blood Vessels
Atherosclerosis
Oxidative Stress
Transcription Factors
Paramagnetic resonance
Assays
Pharmacology
Pressure
Modulation

Keywords

  • Cyclic stretch
  • Endothelium
  • Hemodynamic
  • Protein kinase C
  • Superoxide anion

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Cyclic strain induces reactive oxygen species production via an endothelial NAD(P)H oxidase. / Saito, Hidetsugu; Tada, Shinichiro; Ebinuma, Hirotoshi; Wakabayashi, Kanji; Takagi, Tamako; Saito, Yoshimasa; Nakamoto, Nobuhiro; Kurita, Satoshi; Ishii, Hiromasa.

In: Journal of Cellular Biochemistry, Vol. 81, No. SUPPL. 36, 2001, p. 99-106.

Research output: Contribution to journalArticle

Saito, Hidetsugu ; Tada, Shinichiro ; Ebinuma, Hirotoshi ; Wakabayashi, Kanji ; Takagi, Tamako ; Saito, Yoshimasa ; Nakamoto, Nobuhiro ; Kurita, Satoshi ; Ishii, Hiromasa. / Cyclic strain induces reactive oxygen species production via an endothelial NAD(P)H oxidase. In: Journal of Cellular Biochemistry. 2001 ; Vol. 81, No. SUPPL. 36. pp. 99-106.
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