Cystathionine β-synthase and PGRMC1 as CO sensors

Yasuaki Kabe, Takehiro Yamamoto, Mayumi Kajimura, Yuki Sugiura, Ikko Koike, Mitsuyo Ohmura, Takashi Nakamura, Yasuhito Tokumoto, Hitoshi Tsugawa, Hiroshi Handa, Takuya Kobayashi, Makoto Suematsu

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

Heme oxygenase (HO) is a mono-oxygenase utilizing heme and molecular oxygen (O2) as substrates to generate biliverdin-IXα and carbon monoxide (CO). HO-1 is inducible under stress conditions, while HO-2 is constitutive. A balance between heme and CO was shown to regulate cell death and survival in many experimental models. However, direct molecular targets to which CO binds to regulate cellular functions remained to be fully examined. We have revealed novel roles of CO-responsive proteins, cystathionine β-synthase (CBS) and progesterone receptor membrane component 1 (PGRMC1), in regulating cellular functions. CBS possesses a prosthetic heme that allows CO binding to inhibit the enzyme activity and to regulate H2S generation and/or protein arginine methylation. On the other hand, in response to heme accumulation in cells, PGRMC1 forms a stable dimer through stacking interactions of two protruding heme molecules. Heme-mediated PGRMC1 dimerization is necessary to interact with EGF receptor and cytochromes P450 that determine cell proliferation and xenobiotic metabolism. Furthermore, CO interferes with PGRMC1 dimerization by dissociating the heme stacking, and thus results in modulation of cell responses. This article reviews the intriguing functions of these two proteins in response to inducible and constitutive levels of CO with their pathophysiological implications.

Original languageEnglish
Pages (from-to)333-344
Number of pages12
JournalFree Radical Biology and Medicine
Volume99
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Cystathionine
Progesterone Receptors
Carbon Monoxide
Heme
Membranes
Sensors
Heme Oxygenase (Decyclizing)
Dimerization
Biliverdine
Proteins
Heme Oxygenase-1
Methylation
Molecular oxygen
Cell proliferation
Enzyme activity
Xenobiotics
Cell death
Prosthetics
Epidermal Growth Factor Receptor
Metabolism

Keywords

  • Cancer chemoresistance
  • CO
  • Cystathionine β-synthase (CBS)
  • HS
  • Neurovascular units
  • Progesterone receptor membrane component 1 (PGRMC1)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Cystathionine β-synthase and PGRMC1 as CO sensors. / Kabe, Yasuaki; Yamamoto, Takehiro; Kajimura, Mayumi; Sugiura, Yuki; Koike, Ikko; Ohmura, Mitsuyo; Nakamura, Takashi; Tokumoto, Yasuhito; Tsugawa, Hitoshi; Handa, Hiroshi; Kobayashi, Takuya; Suematsu, Makoto.

In: Free Radical Biology and Medicine, Vol. 99, 01.10.2016, p. 333-344.

Research output: Contribution to journalReview article

Kabe, Yasuaki ; Yamamoto, Takehiro ; Kajimura, Mayumi ; Sugiura, Yuki ; Koike, Ikko ; Ohmura, Mitsuyo ; Nakamura, Takashi ; Tokumoto, Yasuhito ; Tsugawa, Hitoshi ; Handa, Hiroshi ; Kobayashi, Takuya ; Suematsu, Makoto. / Cystathionine β-synthase and PGRMC1 as CO sensors. In: Free Radical Biology and Medicine. 2016 ; Vol. 99. pp. 333-344.
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