Cysteine proteinases and cystatin C in bronchoalveolar lavage fluid from subjects with subclinical emphysema

K. Takeyabu, T. Betsuyaku, M. Nishimura, A. Yoshioka, M. Tanino, K. Miyamoto, Y. Kawakami

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Abstract

This study examined the role of cysteine proteinases and their inhibitor in the development of emphysema in comparison with neutrophil elastase (NE) complexed with α1-protease inhibitor (NE-α1PI), which was previously demonstrated to be increased in bronchoalveolar lavage (BAL) fluid from subjects with subclinical emphysema. Eight nonsmokers and 31 current smokers with (n=17) and without (n=14) emphysema, as evidenced by lung computed tomographic scans, were studied. The concentrations of immunologically detected cathepsin L and cystatin C, but not cathepsin B, were significantly increased in BAL fluid from the smokers with emphysema compared with those without emphysema, although the activity of cathepsin L, measured using a synthetic substrate and cathepsin L released from cultured alveolar macrophages at 24 h, did not show any significant difference between the two groups. When comparison was made only for the subjects aged <60 yrs, the difference between the two groups disappeared for cathepsin L, but remained for NEα1-PI. There was no significant correlation between the level of cathepsin L and that of NE-α1-PI in BAL fluid from the subjects with emphysema. In conclusion, increased levels of cathepsin L and cystatin C were demonstrated in bronchoalveolar lavage fluid from subjects with subclinical emphysema. However, the roles of cathepsin L and neutrophil elastase in the development of emphysema may vary between subjects and between the young and the old.

Original languageEnglish
Pages (from-to)1033-1039
Number of pages7
JournalEuropean Respiratory Journal
Volume12
Issue number5
DOIs
Publication statusPublished - 1998 Nov

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Keywords

  • Alveolar macrophages
  • Bronchoalveolar lavage
  • Cathepsin L
  • Cystatin C
  • Neutrophil elastase
  • Pulmonary emphysema

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Takeyabu, K., Betsuyaku, T., Nishimura, M., Yoshioka, A., Tanino, M., Miyamoto, K., & Kawakami, Y. (1998). Cysteine proteinases and cystatin C in bronchoalveolar lavage fluid from subjects with subclinical emphysema. European Respiratory Journal, 12(5), 1033-1039. https://doi.org/10.1183/09031936.98.12051033