Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines

Fusako Nagai, Yasuko Hiyoshi, Kumiko Sugimachi, Hiro Omi Tamura

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

To explore the physiological roles of cytochrome P450 (CYP) in peripheral blood cells, we examined which isoforms of CYP families were expressed in human myeloid leukemia cell lines (U937, HL-60 and K562) and lymphoid cell lines (BALL-1, MOLT-4 and Jurkat) by RT-PCR. We observed relatively high expression of CYP1A1, CYP1B1, CYP2A6, CYP2A7, CYP2D6, and CYP2E1 in all cell types, but CYP2A13 and CYP2C9 expression was not detected. Expressions of aryl hydrocarbon (Ah) receptor and Ah receptor nuclear translocator (ARNT), which mediate induction of the CYP1 family, were also detected in all cell types. Cell-type specific expression of CYP3A4 and CYP3A5 was observed in MOLT-4 and K562 cells. Weak, but significant, expression of CYP3A7 was detected in K562 cells. The profile of CYP expression in the culture cells reported here provides information that furthers our understanding of the physiological roles of CYP enzymes in human blood cells.

Original languageEnglish
Pages (from-to)383-385
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume25
Issue number3
DOIs
Publication statusPublished - 2002 Mar 1

Keywords

  • Cytochrome P450
  • HL-60
  • Jurkat
  • K562
  • MOLT-4
  • U937

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines'. Together they form a unique fingerprint.

  • Cite this