Cytochrome P450 expression in rat ocular tissues and its induction by phenobarbital

Hiroyuki Tanaka, Ikuko Hirayama, Makoto Takehana, Shizuko Kobayashi, Hiroomi Tamura

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Abstract

Composition and inducibility of cytochrome P450 (CYP) in ocular tissues were investigated using reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot techniques. Composition of ocular CYPs is more restricted than that of hepatic forms. High levels of CYP2B1/2 and reduced levels of CYP2C11 expression were detected in rat ocular tissues. Phenobarbital induced CYP2B1/2 expression in the lens but not in the rest of the eye, whereas CYP2C11 induction was observed in both regions. In addition, CYP2B1/2 proteins were found to be prominent in the retina. Our data indicate eye-specific regulation of CYP expression.

Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalJournal of Health Science
Volume48
Issue number4
DOIs
Publication statusPublished - 2002 Aug

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Keywords

  • Cytochrome P450
  • Lens
  • Ocular tissue

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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