Cytochrome P450 expression in rat ocular tissues and its induction by phenobarbital

Hiroyuki Tanaka, Ikuko Hirayama, Makoto Takehana, Shizuko Kobayashi, Hiroomi Tamura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Composition and inducibility of cytochrome P450 (CYP) in ocular tissues were investigated using reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot techniques. Composition of ocular CYPs is more restricted than that of hepatic forms. High levels of CYP2B1/2 and reduced levels of CYP2C11 expression were detected in rat ocular tissues. Phenobarbital induced CYP2B1/2 expression in the lens but not in the rest of the eye, whereas CYP2C11 induction was observed in both regions. In addition, CYP2B1/2 proteins were found to be prominent in the retina. Our data indicate eye-specific regulation of CYP expression.

Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalJournal of Health Science
Volume48
Issue number4
DOIs
Publication statusPublished - 2002 Aug

Fingerprint

Cytochrome P-450 CYP2B1
Phenobarbital
Cytochrome P-450 Enzyme System
Rats
Tissue
Polymerase chain reaction
Transcription
Chemical analysis
Lenses
Reverse Transcription
Retina
Proteins
Polymerase Chain Reaction
Liver

Keywords

  • Cytochrome P450
  • Lens
  • Ocular tissue

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Cytochrome P450 expression in rat ocular tissues and its induction by phenobarbital. / Tanaka, Hiroyuki; Hirayama, Ikuko; Takehana, Makoto; Kobayashi, Shizuko; Tamura, Hiroomi.

In: Journal of Health Science, Vol. 48, No. 4, 08.2002, p. 346-349.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Hirayama, Ikuko ; Takehana, Makoto ; Kobayashi, Shizuko ; Tamura, Hiroomi. / Cytochrome P450 expression in rat ocular tissues and its induction by phenobarbital. In: Journal of Health Science. 2002 ; Vol. 48, No. 4. pp. 346-349.
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