Cytochrome P450-like Substrate Oxidation Catalyzed by Cytochrome c and Immobilized Cytochrome c

R. Akasaka, Tadahiko Mashino, M. Hirobe

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cytochrome c (cyt.c) was shown to catalyze cytochrome P450 (P450)-like oxidative reactions, such as N-, and 0-demethylation, S-oxidation, and epoxidation of olefins. A more detailed examination showed that (i) N-methylcarbazole and thioanisole oxidation with H218O2 catalyzed by cyt.c resulted in introduction of 18O into the product, and (ii) during the epoxidation of cis-stilbene catalyzed by cyt.c, the stereochemistry of the substrate was retained and 18O was introduced when H218O2 was used as an oxidant. These results show that cyt.c catalyzed N-demethylation, S-oxidation, and epoxidation in the same manner as P450. To utilize these P450-like reactivities effectively, cyt.c was immobilized on poly-γ-methyl-L-glutamate. Up to 99% of the cyt.c used was immobilized. This immobilized cyt.c catalyzed N-demethylation, S-oxidation, and epoxidation in the same manner as both P450 and free cyt.c, and the activities of these reactions were increased by the immobilization. In N-demethylation of N,N-dimethylaniline with cumene hydroperoxide (CHP) catalyzed by cyt.c, the Vmax for CHP was increased by 4.4-fold by the immobilization of the enzyme, while the Km remained unchanged. Since P450 is involved in the metabolism of many xenobiotics, the above results suggest that immobilized cyt.c may be useful in drug metabolism research.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume301
Issue number2
DOIs
Publication statusPublished - 1993 Mar
Externally publishedYes

Fingerprint

Epoxidation
Cytochromes c
Immobilization
Cytochrome P-450 Enzyme System
Stilbenes
Oxidation
Alkenes
Xenobiotics
Substrates
Oxidants
Metabolism
Glutamic Acid
Stereochemistry
Enzymes
Research
Pharmaceutical Preparations
cumene hydroperoxide
methylphenylsulfide
N-methylcarbazole
N,N-dimethylaniline

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Cytochrome P450-like Substrate Oxidation Catalyzed by Cytochrome c and Immobilized Cytochrome c. / Akasaka, R.; Mashino, Tadahiko; Hirobe, M.

In: Archives of Biochemistry and Biophysics, Vol. 301, No. 2, 03.1993, p. 355-360.

Research output: Contribution to journalArticle

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