Cytogenetic analysis of spontaneously discharged products of conception by array-based comparative genomic hybridization

Nobuaki Ozawa, Haruhiko Sago, Kentaro Matsuoka, Tetsuo Maruyama, Ohsuke Migita, Yoshinori Aizu, Johji Inazawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Cytogenetic analysis of products of conception (POC) is essential for the management of recurrent pregnancy loss (RPL), but the currently-performed G-banding method is not necessarily applicable to spontaneously discharged POC because of poor quality for culture. We analyzed the karyotypes of 15 spontaneously discharged POC by array-based comparative genomic hybridization (array-CGH). Results: All specimens were successfully analyzed and 10 cases had abnormal results: gain in copy number (n = 7) and loss in copy number (n = 3). Most of them were estimated to be whole chromosome aneuploidy, whereas one case was compatible with microdeletion. Two cases were suspected to be male diploid contaminated by maternal DNA or triploid because of the unsatisfactory signal patterns on X/Y chromosomes. Two of three cases with normal female DNA pattern were identified to be contaminated with maternal DNA by the additional analysis of short tandem repeats. Conclusions: Given the potential to analyze non-viable POC specimens, array-CGH is a feasible cytogenetic tool for women, in particular, with a history of RPL who desire non-surgical or expectant management of miscarriages and/or a thorough investigation on the cause for recurrent miscarriage, although it needs to take into account high incidence of maternal contamination in spontaneously discharged POC.

Original languageEnglish
Article number874
JournalSpringerPlus
Volume5
Issue number1
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Comparative Genomic Hybridization
Cytogenetic Analysis
Mothers
DNA
Triploidy
Habitual Abortion
Reproductive History
Y Chromosome
X Chromosome
Aneuploidy
Spontaneous Abortion
Diploidy
Karyotype
Cytogenetics
Microsatellite Repeats
Chromosomes
Pregnancy
Incidence

Keywords

  • Array-based comparative genomic hybridization (array-CGH)
  • Chromosomal abnormality
  • Products of conception (POC)
  • Recurrent pregnancy loss (RPL)
  • Short tandem repeat (STR)

ASJC Scopus subject areas

  • General

Cite this

Cytogenetic analysis of spontaneously discharged products of conception by array-based comparative genomic hybridization. / Ozawa, Nobuaki; Sago, Haruhiko; Matsuoka, Kentaro; Maruyama, Tetsuo; Migita, Ohsuke; Aizu, Yoshinori; Inazawa, Johji.

In: SpringerPlus, Vol. 5, No. 1, 874, 01.12.2016.

Research output: Contribution to journalArticle

Ozawa, Nobuaki ; Sago, Haruhiko ; Matsuoka, Kentaro ; Maruyama, Tetsuo ; Migita, Ohsuke ; Aizu, Yoshinori ; Inazawa, Johji. / Cytogenetic analysis of spontaneously discharged products of conception by array-based comparative genomic hybridization. In: SpringerPlus. 2016 ; Vol. 5, No. 1.
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