Cytokine-induced selective increase of high-molecular-weight bFGF isoforms and their subcellular kinetics in cultured rat hippocampal astrocytes

Hiroyuki Kamiguchi, Kazunari Yoshida, Hirooki Wakamoto, Makoto Inaba, Hikaru Sasaki, Mitsuhiro Otani, Shigeo Toya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and epidermal growth factor (EGF) are probable factors responsible for up-regulation of basic fibroblast growth factor (bFGF) expression in reactive astrocytes following brain damage, however the effect of these cytokines on the expression of each bFGF-isoform has not been elucidated. Western blot analysis revealed the expression of 18, 22 and 24-kD hFGF isoforms in cultured rat hippocampal astrocytes, and the expression of high molecular weight (HMW)-isoforms (22 and 24-kD isoforms) but not of 18-kD isoform was selectively increased by cytokines. Immunofluorescent analysis demonstrated that bFGF content in the cytoplasm of astrocytes is initially increased by cytokines followed by nuclear targeting and localization in agreement with the previous evidence that HMW-isoforms possess a nuclear targeting signal. The present results suggest the important role of HMW-bFGF isoforms in the response of nervous tissue to injury.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalNeurochemical Research
Volume21
Issue number6
DOIs
Publication statusPublished - 1996 Jun 1

Keywords

  • Basic fibroblast growth factor
  • astrocyte
  • epidermal growth factor
  • interleukin-1β
  • isoform
  • tumor necrosis factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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