Cytokine Levels in the Aqueous Humor Are Associated With Corneal Thickness in Eyes With Bullous Keratopathy

Natsume Suzuki, Takefumi Yamaguchi, Shinsuke Shibata, Toshihiro Nagai, Hisashi Noma, Kazuo Tsubota, Jun Shimazaki

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3 Citations (Scopus)


Purpose: We sought to investigate the association between the severity of bullous keratopathy and proinflammatory cytokine levels in the aqueous humor (AqH). Design: Cross-sectional study. Methods: This study included a total of 95 eyes: 62 with bullous keratopathy and 33 that underwent cataract surgery. Central corneal thickness (CCT) and central corneal volume within 4 and 6 mm (CCV4mm and CCV6mm, respectively) were determined using anterior segment optical coherence tomography. A total of 95 AqH samples were collected at the beginning of surgery. The levels of cytokines (interleukins [ILs]-1α -1β -4, -6, -8, -10, -12p70, -13, -17A, interferon [IFN]-α IFN-γ monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 [sICAM-1]) in the AqH were measured using multiplex beads immunoassay. We evaluated the correlation among AqH cytokine levels, CCT, CCV4mm, and CCV6mm in eyes with bullous keratopathy. Results: The levels of protein, ILs-4, -6, -8, -10, -12p70, and -17A, MCP-1, IFN-γ E-selectin, P-selectin, and sICAM-1 were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P <.0025). CCT was significantly correlated with the levels of IL-13 (r = 0.551, P =.0009) and sICAM-1 (r = 0.448, P =.0005). CCV4mm was significantly correlated with the levels of IL-13 (r = 0.514, P =.0022) and sICAM-1 (r = 0.404, P =.0019). CCV6mm was significantly correlated with the level of sICAM-1 (r = 0.459, P =.0003). Conclusion: The severity of corneal edema in eyes with bullous keratopathy was associated with the levels of specific cytokines in the AqH.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalAmerican Journal of Ophthalmology
Publication statusPublished - 2019 Feb 1


ASJC Scopus subject areas

  • Ophthalmology

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