Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema

Sadatomo Tasaka, Kosuke Mizoguchi, Yohei Funatsu, Ho Namkoong, Wakako Yamasawa, Makoto Ishii, Naoki Hasegawa, Tomoko Betsuyaku

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background and objective: Combined pulmonary fibrosis and emphysema (CPFE) is characterized by upper lobe emphysema together with lower lobe fibrosis. The aim of this study was to examine whether cytokine levels in the alveolar space are associated with emphysematous changes superimposed on pulmonary fibrosis. Methods: Consecutive patients (n = 102), diagnosed with pulmonary fibrosis were retrospectively evaluated. Cytokine levels and differential cell counts in bronchoalveolar lavage (BAL) fluid, pulmonary function, computed tomography (CT) scores and levels of serum markers were compared between patients with or without emphysema. Results: Among the 102 patients (14 females, mean age 68 years), 38 (37%) had evidence of upper lobe emphysema on computed tomography (CT). Levels of epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) and interleukin (IL)-8/CXCL8 in BAL fluid were significantly higher in patients with emphysema. Vital capacity (VC, % predicted) was greater, and ratio of forced expiratory volume in 1 s/forced vital capacity and diffusing capacity of carbon monoxide (DLCO)/alveolar volume (VA) were lower in patients with emphysema. CXCL8 and CXCL5 levels were associated with percentage or absolute numbers of neutrophils in BAL fluid. In addition, CXCL8 levels were inversely correlated with VC and DLCO/VA, and positively correlated with composite physiological index (CPI) and the extent of areas of low attenuation on CT. Conclusions: Increased CXC chemokine levels in the airspaces may be associated with emphysematous lung changes in patients with pulmonary fibrosis. The levels of various inflammatory mediators in bronchoalveolar lavage fluid were measured in patients with combined pulmonary fibrosis and emphysema. In patients with pulmonary fibrosis, elevated concentrations of CXC chemokines (CXCL5 and CXCL8) were associated with the development of emphysematous changes, neutrophil accumulation in the alveolar space, and impaired diffusing capacity.

Original languageEnglish
Pages (from-to)814-820
Number of pages7
JournalRespirology
Volume17
Issue number5
DOIs
Publication statusPublished - 2012 Jul

Fingerprint

Pulmonary Emphysema
Pulmonary Fibrosis
Bronchoalveolar Lavage Fluid
Cytokines
Emphysema
CXC Chemokines
Neutrophils
Vital Capacity
Tomography
Interleukin-8
Chemokine CXCL5
Lung
Forced Expiratory Volume
Carbon Monoxide
Fibrosis
Cell Count
Biomarkers

Keywords

  • bronchoalveolar lavage
  • chemokine
  • combined pulmonary fibrosis and emphysema
  • computed tomography
  • idiopathic pulmonary fibrosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema. / Tasaka, Sadatomo; Mizoguchi, Kosuke; Funatsu, Yohei; Namkoong, Ho; Yamasawa, Wakako; Ishii, Makoto; Hasegawa, Naoki; Betsuyaku, Tomoko.

In: Respirology, Vol. 17, No. 5, 07.2012, p. 814-820.

Research output: Contribution to journalArticle

Tasaka, Sadatomo ; Mizoguchi, Kosuke ; Funatsu, Yohei ; Namkoong, Ho ; Yamasawa, Wakako ; Ishii, Makoto ; Hasegawa, Naoki ; Betsuyaku, Tomoko. / Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema. In: Respirology. 2012 ; Vol. 17, No. 5. pp. 814-820.
@article{d6b0c81c1e2e42bbb2e4b83021cdbb2d,
title = "Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema",
abstract = "Background and objective: Combined pulmonary fibrosis and emphysema (CPFE) is characterized by upper lobe emphysema together with lower lobe fibrosis. The aim of this study was to examine whether cytokine levels in the alveolar space are associated with emphysematous changes superimposed on pulmonary fibrosis. Methods: Consecutive patients (n = 102), diagnosed with pulmonary fibrosis were retrospectively evaluated. Cytokine levels and differential cell counts in bronchoalveolar lavage (BAL) fluid, pulmonary function, computed tomography (CT) scores and levels of serum markers were compared between patients with or without emphysema. Results: Among the 102 patients (14 females, mean age 68 years), 38 (37{\%}) had evidence of upper lobe emphysema on computed tomography (CT). Levels of epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) and interleukin (IL)-8/CXCL8 in BAL fluid were significantly higher in patients with emphysema. Vital capacity (VC, {\%} predicted) was greater, and ratio of forced expiratory volume in 1 s/forced vital capacity and diffusing capacity of carbon monoxide (DLCO)/alveolar volume (VA) were lower in patients with emphysema. CXCL8 and CXCL5 levels were associated with percentage or absolute numbers of neutrophils in BAL fluid. In addition, CXCL8 levels were inversely correlated with VC and DLCO/VA, and positively correlated with composite physiological index (CPI) and the extent of areas of low attenuation on CT. Conclusions: Increased CXC chemokine levels in the airspaces may be associated with emphysematous lung changes in patients with pulmonary fibrosis. The levels of various inflammatory mediators in bronchoalveolar lavage fluid were measured in patients with combined pulmonary fibrosis and emphysema. In patients with pulmonary fibrosis, elevated concentrations of CXC chemokines (CXCL5 and CXCL8) were associated with the development of emphysematous changes, neutrophil accumulation in the alveolar space, and impaired diffusing capacity.",
keywords = "bronchoalveolar lavage, chemokine, combined pulmonary fibrosis and emphysema, computed tomography, idiopathic pulmonary fibrosis",
author = "Sadatomo Tasaka and Kosuke Mizoguchi and Yohei Funatsu and Ho Namkoong and Wakako Yamasawa and Makoto Ishii and Naoki Hasegawa and Tomoko Betsuyaku",
year = "2012",
month = "7",
doi = "10.1111/j.1440-1843.2012.02182.x",
language = "English",
volume = "17",
pages = "814--820",
journal = "Respirology",
issn = "1323-7799",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema

AU - Tasaka, Sadatomo

AU - Mizoguchi, Kosuke

AU - Funatsu, Yohei

AU - Namkoong, Ho

AU - Yamasawa, Wakako

AU - Ishii, Makoto

AU - Hasegawa, Naoki

AU - Betsuyaku, Tomoko

PY - 2012/7

Y1 - 2012/7

N2 - Background and objective: Combined pulmonary fibrosis and emphysema (CPFE) is characterized by upper lobe emphysema together with lower lobe fibrosis. The aim of this study was to examine whether cytokine levels in the alveolar space are associated with emphysematous changes superimposed on pulmonary fibrosis. Methods: Consecutive patients (n = 102), diagnosed with pulmonary fibrosis were retrospectively evaluated. Cytokine levels and differential cell counts in bronchoalveolar lavage (BAL) fluid, pulmonary function, computed tomography (CT) scores and levels of serum markers were compared between patients with or without emphysema. Results: Among the 102 patients (14 females, mean age 68 years), 38 (37%) had evidence of upper lobe emphysema on computed tomography (CT). Levels of epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) and interleukin (IL)-8/CXCL8 in BAL fluid were significantly higher in patients with emphysema. Vital capacity (VC, % predicted) was greater, and ratio of forced expiratory volume in 1 s/forced vital capacity and diffusing capacity of carbon monoxide (DLCO)/alveolar volume (VA) were lower in patients with emphysema. CXCL8 and CXCL5 levels were associated with percentage or absolute numbers of neutrophils in BAL fluid. In addition, CXCL8 levels were inversely correlated with VC and DLCO/VA, and positively correlated with composite physiological index (CPI) and the extent of areas of low attenuation on CT. Conclusions: Increased CXC chemokine levels in the airspaces may be associated with emphysematous lung changes in patients with pulmonary fibrosis. The levels of various inflammatory mediators in bronchoalveolar lavage fluid were measured in patients with combined pulmonary fibrosis and emphysema. In patients with pulmonary fibrosis, elevated concentrations of CXC chemokines (CXCL5 and CXCL8) were associated with the development of emphysematous changes, neutrophil accumulation in the alveolar space, and impaired diffusing capacity.

AB - Background and objective: Combined pulmonary fibrosis and emphysema (CPFE) is characterized by upper lobe emphysema together with lower lobe fibrosis. The aim of this study was to examine whether cytokine levels in the alveolar space are associated with emphysematous changes superimposed on pulmonary fibrosis. Methods: Consecutive patients (n = 102), diagnosed with pulmonary fibrosis were retrospectively evaluated. Cytokine levels and differential cell counts in bronchoalveolar lavage (BAL) fluid, pulmonary function, computed tomography (CT) scores and levels of serum markers were compared between patients with or without emphysema. Results: Among the 102 patients (14 females, mean age 68 years), 38 (37%) had evidence of upper lobe emphysema on computed tomography (CT). Levels of epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) and interleukin (IL)-8/CXCL8 in BAL fluid were significantly higher in patients with emphysema. Vital capacity (VC, % predicted) was greater, and ratio of forced expiratory volume in 1 s/forced vital capacity and diffusing capacity of carbon monoxide (DLCO)/alveolar volume (VA) were lower in patients with emphysema. CXCL8 and CXCL5 levels were associated with percentage or absolute numbers of neutrophils in BAL fluid. In addition, CXCL8 levels were inversely correlated with VC and DLCO/VA, and positively correlated with composite physiological index (CPI) and the extent of areas of low attenuation on CT. Conclusions: Increased CXC chemokine levels in the airspaces may be associated with emphysematous lung changes in patients with pulmonary fibrosis. The levels of various inflammatory mediators in bronchoalveolar lavage fluid were measured in patients with combined pulmonary fibrosis and emphysema. In patients with pulmonary fibrosis, elevated concentrations of CXC chemokines (CXCL5 and CXCL8) were associated with the development of emphysematous changes, neutrophil accumulation in the alveolar space, and impaired diffusing capacity.

KW - bronchoalveolar lavage

KW - chemokine

KW - combined pulmonary fibrosis and emphysema

KW - computed tomography

KW - idiopathic pulmonary fibrosis

UR - http://www.scopus.com/inward/record.url?scp=84862993735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862993735&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1843.2012.02182.x

DO - 10.1111/j.1440-1843.2012.02182.x

M3 - Article

C2 - 22515655

AN - SCOPUS:84862993735

VL - 17

SP - 814

EP - 820

JO - Respirology

JF - Respirology

SN - 1323-7799

IS - 5

ER -