Cytokine profiles of bronchoalveolar lavage fluid in patients with pneumocystis pneumonia

Sadatomo Tasaka, Seiki Kobayashi, Hirofumi Kamata, Yoshifumi Kimizuka, Hiroshi Fujiwara, Yohei Funatsu, Kosuke Mizoguchi, Makoto Ishii, Tsutomu Takeuchi, Naoki Hasegawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The clinical features of PCP differ according to the factors responsible for the predisposing immunosuppression. Although the diagnosis of PCP often requires BAL, the profiles of the inflammatory mediators in the BAL fluid are not thoroughly documented. The aim of the current study was to characterize the profiles of inflammatory mediators in BAL fluid during PCP in patients with underlying autoimmune diseases, malignancies, or AIDS. The medical records of 14 patients with autoimmune diseases, 10 with malignancies, and 8 with AIDS, all of whom had been diagnosed with PCP by microscopic examination of BAL fluid, were reviewed. The concentrations of TNF-α, MCP-1, HMGB1, IL-8, IL-6, IL-10, and IFN-γ in the BAL fluid that had been obtained for the diagnosis of PCP were measured. The concentrations of MCP-1, IL-8, and IL-6 differed according to the underlying disease, tending to be higher in patients with autoimmune diseases and lower in those with AIDS. The concentrations of HMGB1, IL-8, and IL-6 were positively correlated with the proportion of neutrophils in BAL fluid and inversely with the oxygenation index. Although the serum concentrations of CRP and LDH were positively correlated with those of IL-8 and MCP-1, none of the mediators in BAL fluid was correlated with the serum β-D-glucan concentration. The production of inflammatory mediators in the lung differed between the patient groups with different underlying disorders. The modest upregulation of IL-8 and IL-6 might be associated with the milder clinical manifestations of PCP in AIDS patients.

Original languageEnglish
Pages (from-to)425-433
Number of pages9
JournalMicrobiology and Immunology
Volume54
Issue number7
DOIs
Publication statusPublished - 2010 Jul

Fingerprint

Dimercaprol
Pneumocystis Pneumonia
Bronchoalveolar Lavage Fluid
Interleukin-8
Cytokines
Interleukin-6
Acquired Immunodeficiency Syndrome
HMGB1 Protein
Autoimmune Diseases
Serum
Causality
Interleukin-10
Immunosuppression
Medical Records
Neoplasms
Neutrophils
Up-Regulation
Lung

Keywords

  • Acquired immunodeficiency syndrome
  • Bronchoalveolar lavage
  • Cytokine
  • Pneumocystis pneumonia

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Cytokine profiles of bronchoalveolar lavage fluid in patients with pneumocystis pneumonia. / Tasaka, Sadatomo; Kobayashi, Seiki; Kamata, Hirofumi; Kimizuka, Yoshifumi; Fujiwara, Hiroshi; Funatsu, Yohei; Mizoguchi, Kosuke; Ishii, Makoto; Takeuchi, Tsutomu; Hasegawa, Naoki.

In: Microbiology and Immunology, Vol. 54, No. 7, 07.2010, p. 425-433.

Research output: Contribution to journalArticle

Tasaka, Sadatomo ; Kobayashi, Seiki ; Kamata, Hirofumi ; Kimizuka, Yoshifumi ; Fujiwara, Hiroshi ; Funatsu, Yohei ; Mizoguchi, Kosuke ; Ishii, Makoto ; Takeuchi, Tsutomu ; Hasegawa, Naoki. / Cytokine profiles of bronchoalveolar lavage fluid in patients with pneumocystis pneumonia. In: Microbiology and Immunology. 2010 ; Vol. 54, No. 7. pp. 425-433.
@article{c0f583a35e144da29cd715cf36594d52,
title = "Cytokine profiles of bronchoalveolar lavage fluid in patients with pneumocystis pneumonia",
abstract = "The clinical features of PCP differ according to the factors responsible for the predisposing immunosuppression. Although the diagnosis of PCP often requires BAL, the profiles of the inflammatory mediators in the BAL fluid are not thoroughly documented. The aim of the current study was to characterize the profiles of inflammatory mediators in BAL fluid during PCP in patients with underlying autoimmune diseases, malignancies, or AIDS. The medical records of 14 patients with autoimmune diseases, 10 with malignancies, and 8 with AIDS, all of whom had been diagnosed with PCP by microscopic examination of BAL fluid, were reviewed. The concentrations of TNF-α, MCP-1, HMGB1, IL-8, IL-6, IL-10, and IFN-γ in the BAL fluid that had been obtained for the diagnosis of PCP were measured. The concentrations of MCP-1, IL-8, and IL-6 differed according to the underlying disease, tending to be higher in patients with autoimmune diseases and lower in those with AIDS. The concentrations of HMGB1, IL-8, and IL-6 were positively correlated with the proportion of neutrophils in BAL fluid and inversely with the oxygenation index. Although the serum concentrations of CRP and LDH were positively correlated with those of IL-8 and MCP-1, none of the mediators in BAL fluid was correlated with the serum β-D-glucan concentration. The production of inflammatory mediators in the lung differed between the patient groups with different underlying disorders. The modest upregulation of IL-8 and IL-6 might be associated with the milder clinical manifestations of PCP in AIDS patients.",
keywords = "Acquired immunodeficiency syndrome, Bronchoalveolar lavage, Cytokine, Pneumocystis pneumonia",
author = "Sadatomo Tasaka and Seiki Kobayashi and Hirofumi Kamata and Yoshifumi Kimizuka and Hiroshi Fujiwara and Yohei Funatsu and Kosuke Mizoguchi and Makoto Ishii and Tsutomu Takeuchi and Naoki Hasegawa",
year = "2010",
month = "7",
doi = "10.1111/j.1348-0421.2010.00229.x",
language = "English",
volume = "54",
pages = "425--433",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "7",

}

TY - JOUR

T1 - Cytokine profiles of bronchoalveolar lavage fluid in patients with pneumocystis pneumonia

AU - Tasaka, Sadatomo

AU - Kobayashi, Seiki

AU - Kamata, Hirofumi

AU - Kimizuka, Yoshifumi

AU - Fujiwara, Hiroshi

AU - Funatsu, Yohei

AU - Mizoguchi, Kosuke

AU - Ishii, Makoto

AU - Takeuchi, Tsutomu

AU - Hasegawa, Naoki

PY - 2010/7

Y1 - 2010/7

N2 - The clinical features of PCP differ according to the factors responsible for the predisposing immunosuppression. Although the diagnosis of PCP often requires BAL, the profiles of the inflammatory mediators in the BAL fluid are not thoroughly documented. The aim of the current study was to characterize the profiles of inflammatory mediators in BAL fluid during PCP in patients with underlying autoimmune diseases, malignancies, or AIDS. The medical records of 14 patients with autoimmune diseases, 10 with malignancies, and 8 with AIDS, all of whom had been diagnosed with PCP by microscopic examination of BAL fluid, were reviewed. The concentrations of TNF-α, MCP-1, HMGB1, IL-8, IL-6, IL-10, and IFN-γ in the BAL fluid that had been obtained for the diagnosis of PCP were measured. The concentrations of MCP-1, IL-8, and IL-6 differed according to the underlying disease, tending to be higher in patients with autoimmune diseases and lower in those with AIDS. The concentrations of HMGB1, IL-8, and IL-6 were positively correlated with the proportion of neutrophils in BAL fluid and inversely with the oxygenation index. Although the serum concentrations of CRP and LDH were positively correlated with those of IL-8 and MCP-1, none of the mediators in BAL fluid was correlated with the serum β-D-glucan concentration. The production of inflammatory mediators in the lung differed between the patient groups with different underlying disorders. The modest upregulation of IL-8 and IL-6 might be associated with the milder clinical manifestations of PCP in AIDS patients.

AB - The clinical features of PCP differ according to the factors responsible for the predisposing immunosuppression. Although the diagnosis of PCP often requires BAL, the profiles of the inflammatory mediators in the BAL fluid are not thoroughly documented. The aim of the current study was to characterize the profiles of inflammatory mediators in BAL fluid during PCP in patients with underlying autoimmune diseases, malignancies, or AIDS. The medical records of 14 patients with autoimmune diseases, 10 with malignancies, and 8 with AIDS, all of whom had been diagnosed with PCP by microscopic examination of BAL fluid, were reviewed. The concentrations of TNF-α, MCP-1, HMGB1, IL-8, IL-6, IL-10, and IFN-γ in the BAL fluid that had been obtained for the diagnosis of PCP were measured. The concentrations of MCP-1, IL-8, and IL-6 differed according to the underlying disease, tending to be higher in patients with autoimmune diseases and lower in those with AIDS. The concentrations of HMGB1, IL-8, and IL-6 were positively correlated with the proportion of neutrophils in BAL fluid and inversely with the oxygenation index. Although the serum concentrations of CRP and LDH were positively correlated with those of IL-8 and MCP-1, none of the mediators in BAL fluid was correlated with the serum β-D-glucan concentration. The production of inflammatory mediators in the lung differed between the patient groups with different underlying disorders. The modest upregulation of IL-8 and IL-6 might be associated with the milder clinical manifestations of PCP in AIDS patients.

KW - Acquired immunodeficiency syndrome

KW - Bronchoalveolar lavage

KW - Cytokine

KW - Pneumocystis pneumonia

UR - http://www.scopus.com/inward/record.url?scp=77954475111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954475111&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.2010.00229.x

DO - 10.1111/j.1348-0421.2010.00229.x

M3 - Article

VL - 54

SP - 425

EP - 433

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 7

ER -