Cytokines regulate c-Met expression in cultured astrocytes

Kenji Shimazaki; Kazunari Yoshida; Yuji Hirose; Hisatsugu Ishimori; Makoto Katayama; Takeshi Kawase

doi:10.1016/S0006-8993(02)03975-6

Cytokines regulate c-Met expression in cultured astrocytes

Kenji Shimazaki, Kazunari Yoshida, Yuji Hirose, Hisatsugu Ishimori, Makoto Katayama, Takeshi Kawase

Department of Neurosurgery

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

Original language	English
Pages (from-to)	105-110
Number of pages	6
Journal	Brain Research
Volume	962
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(02)03975-6
Publication status	Published - 2003 Feb 7

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Keywords

Astrocyte
Double immunofluorescent staining
RT-PCR
Regeneration
Western blot analysis
c-Met

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

Cite this

Shimazaki, K., Yoshida, K., Hirose, Y., Ishimori, H., Katayama, M., & Kawase, T. (2003). Cytokines regulate c-Met expression in cultured astrocytes. Brain Research, 962(1-2), 105-110. https://doi.org/10.1016/S0006-8993(02)03975-6

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AU - Kawase, Takeshi

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AB - We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

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Access to Document

10.1016/S0006-8993(02)03975-6