Cytokines regulate c-Met expression in cultured astrocytes

Kenji Shimazaki, Kazunari Yoshida, Yuji Hirose, Hisatsugu Ishimori, Makoto Katayama, Takeshi Kawase

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13 Citations (Scopus)

Abstract

We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalBrain Research
Volume962
Issue number1-2
DOIs
Publication statusPublished - 2003 Feb 7

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Keywords

  • Astrocyte
  • Double immunofluorescent staining
  • RT-PCR
  • Regeneration
  • Western blot analysis
  • c-Met

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Shimazaki, K., Yoshida, K., Hirose, Y., Ishimori, H., Katayama, M., & Kawase, T. (2003). Cytokines regulate c-Met expression in cultured astrocytes. Brain Research, 962(1-2), 105-110. https://doi.org/10.1016/S0006-8993(02)03975-6