Cytokines regulate c-Met expression in cultured astrocytes

Kenji Shimazaki; Kazunari Yoshida; Yuji Hirose; Hisatsugu Ishimori; Makoto Katayama; Takeshi Kawase

doi:10.1016/S0006-8993(02)03975-6

Cytokines regulate c-Met expression in cultured astrocytes

Kenji Shimazaki, Kazunari Yoshida, Yuji Hirose, Hisatsugu Ishimori, Makoto Katayama, Takeshi Kawase

Department of Neurosurgery

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

Original language	English
Pages (from-to)	105-110
Number of pages	6
Journal	Brain Research
Volume	962
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(02)03975-6
Publication status	Published - 2003 Feb 7

Keywords

Astrocyte
Double immunofluorescent staining
RT-PCR
Regeneration
Western blot analysis
c-Met

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Cytokines regulate c-Met expression in cultured astrocytes",

abstract = "We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.",

keywords = "Astrocyte, Double immunofluorescent staining, RT-PCR, Regeneration, Western blot analysis, c-Met",

author = "Kenji Shimazaki and Kazunari Yoshida and Yuji Hirose and Hisatsugu Ishimori and Makoto Katayama and Takeshi Kawase",

note = "Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture, no. 08671615 and no. 10770691.",

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T1 - Cytokines regulate c-Met expression in cultured astrocytes

AU - Shimazaki, Kenji

AU - Yoshida, Kazunari

AU - Hirose, Yuji

AU - Ishimori, Hisatsugu

AU - Katayama, Makoto

AU - Kawase, Takeshi

N1 - Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture, no. 08671615 and no. 10770691.

PY - 2003/2/7

Y1 - 2003/2/7

N2 - We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

AB - We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

KW - Astrocyte

KW - Double immunofluorescent staining

KW - RT-PCR

KW - Regeneration

KW - Western blot analysis

KW - c-Met

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