Cytologic evaluation of conjunctival epithelium using Cytobrush-S: Value of slide preparation by ThinPrep technique

T. K. Kobayashi, M. Ueda, T. Nishino, Y. Ishida, E. Takamura, Kazuo Tsubota

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recent clinical trials have indicated that an automated smear apparatus (ThinPrep process) of sample preparation has great diagnostic sensitivity. In this study, conjunctival brush cytology prepared using the ThinPrep method was applied in ocular surface disorders especially for dry eye status. To assess its diagnostic value in cellular samples, 17 patients with keratoconjunctivitis sicca (KCS) and 10 normal volunteer patients were examined using this technique. Conjunctival cells from normal controls revealed fine chromatin and polyhedral cytoplasm without having keratinized cytoplasm. On the other hand, the cellular samples from KCS revealed increased keratinized cells with pyknotic nuclei. They also contained extremely elongated cells. In KCS patients, the mean number of keratinized cells was significantly higher (34-1 cells/300 cells) than that of the normal control group (0.2 cells/300 cells). In patients with KCS, inflammatory cell counts were also higher than those of normal controls. Conjunctival cytology by means of the ThinPrep obviously deserves additional trials as an adjunct in the cytology of dry eye states, especially in quantitative ocular evaluation for various ocular lesions.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalCytopathology
Volume8
Issue number6
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Keratoconjunctivitis Sicca
Epithelium
Cell Biology
Cytoplasm
Cell Count
Chromatin
Healthy Volunteers
Clinical Trials
Control Groups

Keywords

  • Brush cytology
  • Immunocytochemistry
  • Ocular cytology
  • Quantitative cytology
  • ThinPrep process

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Cytologic evaluation of conjunctival epithelium using Cytobrush-S : Value of slide preparation by ThinPrep technique. / Kobayashi, T. K.; Ueda, M.; Nishino, T.; Ishida, Y.; Takamura, E.; Tsubota, Kazuo.

In: Cytopathology, Vol. 8, No. 6, 1997, p. 381-387.

Research output: Contribution to journalArticle

Kobayashi, TK, Ueda, M, Nishino, T, Ishida, Y, Takamura, E & Tsubota, K 1997, 'Cytologic evaluation of conjunctival epithelium using Cytobrush-S: Value of slide preparation by ThinPrep technique', Cytopathology, vol. 8, no. 6, pp. 381-387.
Kobayashi, T. K. ; Ueda, M. ; Nishino, T. ; Ishida, Y. ; Takamura, E. ; Tsubota, Kazuo. / Cytologic evaluation of conjunctival epithelium using Cytobrush-S : Value of slide preparation by ThinPrep technique. In: Cytopathology. 1997 ; Vol. 8, No. 6. pp. 381-387.
@article{7bb21fee5d8b412794f4edb68c2bca66,
title = "Cytologic evaluation of conjunctival epithelium using Cytobrush-S: Value of slide preparation by ThinPrep technique",
abstract = "Recent clinical trials have indicated that an automated smear apparatus (ThinPrep process) of sample preparation has great diagnostic sensitivity. In this study, conjunctival brush cytology prepared using the ThinPrep method was applied in ocular surface disorders especially for dry eye status. To assess its diagnostic value in cellular samples, 17 patients with keratoconjunctivitis sicca (KCS) and 10 normal volunteer patients were examined using this technique. Conjunctival cells from normal controls revealed fine chromatin and polyhedral cytoplasm without having keratinized cytoplasm. On the other hand, the cellular samples from KCS revealed increased keratinized cells with pyknotic nuclei. They also contained extremely elongated cells. In KCS patients, the mean number of keratinized cells was significantly higher (34-1 cells/300 cells) than that of the normal control group (0.2 cells/300 cells). In patients with KCS, inflammatory cell counts were also higher than those of normal controls. Conjunctival cytology by means of the ThinPrep obviously deserves additional trials as an adjunct in the cytology of dry eye states, especially in quantitative ocular evaluation for various ocular lesions.",
keywords = "Brush cytology, Immunocytochemistry, Ocular cytology, Quantitative cytology, ThinPrep process",
author = "Kobayashi, {T. K.} and M. Ueda and T. Nishino and Y. Ishida and E. Takamura and Kazuo Tsubota",
year = "1997",
language = "English",
volume = "8",
pages = "381--387",
journal = "Cytopathology",
issn = "0956-5507",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Cytologic evaluation of conjunctival epithelium using Cytobrush-S

T2 - Value of slide preparation by ThinPrep technique

AU - Kobayashi, T. K.

AU - Ueda, M.

AU - Nishino, T.

AU - Ishida, Y.

AU - Takamura, E.

AU - Tsubota, Kazuo

PY - 1997

Y1 - 1997

N2 - Recent clinical trials have indicated that an automated smear apparatus (ThinPrep process) of sample preparation has great diagnostic sensitivity. In this study, conjunctival brush cytology prepared using the ThinPrep method was applied in ocular surface disorders especially for dry eye status. To assess its diagnostic value in cellular samples, 17 patients with keratoconjunctivitis sicca (KCS) and 10 normal volunteer patients were examined using this technique. Conjunctival cells from normal controls revealed fine chromatin and polyhedral cytoplasm without having keratinized cytoplasm. On the other hand, the cellular samples from KCS revealed increased keratinized cells with pyknotic nuclei. They also contained extremely elongated cells. In KCS patients, the mean number of keratinized cells was significantly higher (34-1 cells/300 cells) than that of the normal control group (0.2 cells/300 cells). In patients with KCS, inflammatory cell counts were also higher than those of normal controls. Conjunctival cytology by means of the ThinPrep obviously deserves additional trials as an adjunct in the cytology of dry eye states, especially in quantitative ocular evaluation for various ocular lesions.

AB - Recent clinical trials have indicated that an automated smear apparatus (ThinPrep process) of sample preparation has great diagnostic sensitivity. In this study, conjunctival brush cytology prepared using the ThinPrep method was applied in ocular surface disorders especially for dry eye status. To assess its diagnostic value in cellular samples, 17 patients with keratoconjunctivitis sicca (KCS) and 10 normal volunteer patients were examined using this technique. Conjunctival cells from normal controls revealed fine chromatin and polyhedral cytoplasm without having keratinized cytoplasm. On the other hand, the cellular samples from KCS revealed increased keratinized cells with pyknotic nuclei. They also contained extremely elongated cells. In KCS patients, the mean number of keratinized cells was significantly higher (34-1 cells/300 cells) than that of the normal control group (0.2 cells/300 cells). In patients with KCS, inflammatory cell counts were also higher than those of normal controls. Conjunctival cytology by means of the ThinPrep obviously deserves additional trials as an adjunct in the cytology of dry eye states, especially in quantitative ocular evaluation for various ocular lesions.

KW - Brush cytology

KW - Immunocytochemistry

KW - Ocular cytology

KW - Quantitative cytology

KW - ThinPrep process

UR - http://www.scopus.com/inward/record.url?scp=0031460360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031460360&partnerID=8YFLogxK

M3 - Article

C2 - 9439890

AN - SCOPUS:0031460360

VL - 8

SP - 381

EP - 387

JO - Cytopathology

JF - Cytopathology

SN - 0956-5507

IS - 6

ER -