Cytosol-endoplasmic reticulum interplay by Sec61α translocon in polyglutamine-mediated neurotoxicity in Drosophila

Hirotaka Kanuka, Erina Kuranaga, Tetsuo Hiratou, Tatsushi Igaki, Bryce Nelson, Hideyuki Okano, Masayuki Miura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Intracellular deposition of aggregated and ubiquitinated proteins is a prominent cytopathological feature of most neurodegenerative disorders frequently correlated with neural cell death. To elucidate mechanisms in neural cell death and degeneration, we characterized the Drosophila ortholog of Sec61α (DSec61α), a component of the translocon that is involved in both protein import and endoplasmic reticulum-associated degradation. Loss-of-function experiments for Dsec61α revealed that the translocon contributes to expanded polyglutamine-mediated neuronal toxicity, likely resulting from proteasome inhibition and leading to accumulation of ubiquitinated proteins. Taken together, proteasome inhibition by expanded polyglutamine tracts may lead to the accumulation of toxic undegraded proteins normally transported by the Sec61α translocon.

Original languageEnglish
Pages (from-to)11723-11728
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number20
DOIs
Publication statusPublished - 2003 Sep 30

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Ubiquitinated Proteins
Proteasome Endopeptidase Complex
Endoplasmic Reticulum
Cytosol
Drosophila
Cell Death
Endoplasmic Reticulum-Associated Degradation
Poisons
Neurodegenerative Diseases
Proteins
polyglutamine

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cytosol-endoplasmic reticulum interplay by Sec61α translocon in polyglutamine-mediated neurotoxicity in Drosophila. / Kanuka, Hirotaka; Kuranaga, Erina; Hiratou, Tetsuo; Igaki, Tatsushi; Nelson, Bryce; Okano, Hideyuki; Miura, Masayuki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 20, 30.09.2003, p. 11723-11728.

Research output: Contribution to journalArticle

Kanuka, Hirotaka ; Kuranaga, Erina ; Hiratou, Tetsuo ; Igaki, Tatsushi ; Nelson, Bryce ; Okano, Hideyuki ; Miura, Masayuki. / Cytosol-endoplasmic reticulum interplay by Sec61α translocon in polyglutamine-mediated neurotoxicity in Drosophila. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 20. pp. 11723-11728.
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