Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte

Emiyu Ogawa, Sayaka Motohashi, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the myocardial cell necrosis measuring cell lethality with various albumin concentrations against oxidative stress by extracellular photosensitization reaction using talaporfin sodium. We have proposed to induce the photosensitization reaction in interstitial space of myocardium with short drug-light interval to realize the immediate cell damage by myocardial cell membrane for tachyarrhythmia treatment. To understand the effect of the particular extracellular photosensitization reaction in interstitial space, we measured the myocardial cell lethality 2 hours after the photosensitization reaction by WST assay varying the talaporfin sodium concentration, radiant exposure, and wide albumin concentration of 0-15 mg/ml. The cell lethality was decreased with albumin concentration increasing over 85 % in binding ratio between talaporfin sodium and albumin. The cell lethality didn’t change between 0-85% in binding ratio between talaporfin sodium and albumin. The calculated deposited energy to the talaporfin sodium solution was nearly constant of 8.4±1.6 J/well in average varying albumin concentration. We think the cell killing effect by the extracellular photosensitization reaction has a threshold between 85-100% in the binding ratio between talaporfin sodium and albumin in the case of the talaporfin sodium concentration of 40 μg/ml and radiant exposure of 0-40 J/cm<sup>2</sup>.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9321
ISBN (Print)9781628414110
DOIs
Publication statusPublished - 2014
EventOptical Interactions with Tissue and Cells XXVI - San Francisco, United States
Duration: 2015 Feb 82015 Feb 10

Other

OtherOptical Interactions with Tissue and Cells XXVI
CountryUnited States
CitySan Francisco
Period15/2/815/2/10

Fingerprint

Photosensitivity Disorders
Cytotoxicity
albumins
Cardiac Myocytes
Albumins
lethality
Sodium
sodium
cells
interstitials
Cells
myocardium
Oxidative stress
necrosis
Cell membranes
Talaporfin
Tachycardia
Assays
Myocardium
drugs

Keywords

  • Laserphyrin
  • myocardial cell
  • NPe6

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ogawa, E., Motohashi, S., & Arai, T. (2014). Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9321). [93210I] SPIE. https://doi.org/10.1117/12.2078454

Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. / Ogawa, Emiyu; Motohashi, Sayaka; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9321 SPIE, 2014. 93210I.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, E, Motohashi, S & Arai, T 2014, Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9321, 93210I, SPIE, Optical Interactions with Tissue and Cells XXVI, San Francisco, United States, 15/2/8. https://doi.org/10.1117/12.2078454
Ogawa E, Motohashi S, Arai T. Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9321. SPIE. 2014. 93210I https://doi.org/10.1117/12.2078454
Ogawa, Emiyu ; Motohashi, Sayaka ; Arai, Tsunenori. / Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9321 SPIE, 2014.
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