Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte

Emiyu Ogawa, Sayaka Motohashi, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the myocardial cell necrosis measuring cell lethality with various albumin concentrations against oxidative stress by extracellular photosensitization reaction using talaporfin sodium. We have proposed to induce the photosensitization reaction in interstitial space of myocardium with short drug-light interval to realize the immediate cell damage by myocardial cell membrane for tachyarrhythmia treatment. To understand the effect of the particular extracellular photosensitization reaction in interstitial space, we measured the myocardial cell lethality 2 hours after the photosensitization reaction by WST assay varying the talaporfin sodium concentration, radiant exposure, and wide albumin concentration of 0-15 mg/ml. The cell lethality was decreased with albumin concentration increasing over 85 % in binding ratio between talaporfin sodium and albumin. The cell lethality didn’t change between 0-85% in binding ratio between talaporfin sodium and albumin. The calculated deposited energy to the talaporfin sodium solution was nearly constant of 8.4±1.6 J/well in average varying albumin concentration. We think the cell killing effect by the extracellular photosensitization reaction has a threshold between 85-100% in the binding ratio between talaporfin sodium and albumin in the case of the talaporfin sodium concentration of 40 μg/ml and radiant exposure of 0-40 J/cm2.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXVI
EditorsE. Duco Jansen
PublisherSPIE
ISBN (Electronic)9781628414110
DOIs
Publication statusPublished - 2014 Jan 1
EventOptical Interactions with Tissue and Cells XXVI - San Francisco, United States
Duration: 2015 Feb 82015 Feb 10

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9321
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXVI
CountryUnited States
CitySan Francisco
Period15/2/815/2/10

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Keywords

  • Laserphyrin
  • NPe6
  • myocardial cell

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Ogawa, E., Motohashi, S., & Arai, T. (2014). Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte. In E. D. Jansen (Ed.), Optical Interactions with Tissue and Cells XXVI [93210I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9321). SPIE. https://doi.org/10.1117/12.2078454