Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells

Taiko Oda, Noriko Ikeda, Shoko Nakashima, Yoshihiro Sato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalDrug and Chemical Toxicology
Volume25
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Equilenin
Equilin
Cytotoxicity
Cricetulus
Estradiol
Estrogens
Derivatives
Diethylstilbestrol
Aneuploidy
Skeleton
Microtubules
Hydroxyl Radical
Horses
Urine
Plating

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells. / Oda, Taiko; Ikeda, Noriko; Nakashima, Shoko; Sato, Yoshihiro.

In: Drug and Chemical Toxicology, Vol. 25, No. 1, 2002, p. 75-82.

Research output: Contribution to journalArticle

Oda, Taiko ; Ikeda, Noriko ; Nakashima, Shoko ; Sato, Yoshihiro. / Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells. In: Drug and Chemical Toxicology. 2002 ; Vol. 25, No. 1. pp. 75-82.
@article{540acaabf0f24c91bbbc46b4fdcf8fab,
title = "Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells",
abstract = "Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.",
author = "Taiko Oda and Noriko Ikeda and Shoko Nakashima and Yoshihiro Sato",
year = "2002",
doi = "10.1081/DCT-100108473",
language = "English",
volume = "25",
pages = "75--82",
journal = "Drug and Chemical Toxicology",
issn = "0148-0545",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells

AU - Oda, Taiko

AU - Ikeda, Noriko

AU - Nakashima, Shoko

AU - Sato, Yoshihiro

PY - 2002

Y1 - 2002

N2 - Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.

AB - Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.

UR - http://www.scopus.com/inward/record.url?scp=0036166267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036166267&partnerID=8YFLogxK

U2 - 10.1081/DCT-100108473

DO - 10.1081/DCT-100108473

M3 - Article

C2 - 11850971

AN - SCOPUS:0036166267

VL - 25

SP - 75

EP - 82

JO - Drug and Chemical Toxicology

JF - Drug and Chemical Toxicology

SN - 0148-0545

IS - 1

ER -