Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells

Taiko Oda, Motohiro Tanaka, Takuma Sasaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have shown that synthetic and natural estrogens and related compounds inhibit the polymerization of microtubule proteins. In this study, cell growth inhibition by synthetic estrogens and their related compounds was examined by the MTT method using L1210, KB, and NIH-3T3 cells transformed with oncogenes, which are typical screening systems for carcinostatics. [(-)3R]Indenestrol B, a derivative of diethylstilbestrol (DES), which strongly inhibited the polymerization of microtubule proteins, also showed marked inhibition of the growth of KB cells and various oncogene-transformed NIH-3T3 cells. On the other hand, DES and indenestrol A markedly inhibited the growth of L1210 cells, indicating that these compounds exhibit cell-specific inhibitory effects on cell growth. Although the inhibition of cell growth by these compounds was not as strong as that by colchicine, the results clearly indicate the potential of these compounds as carcinostatics.

Original languageEnglish
Pages (from-to)1142-1144
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume24
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Estradiol Congeners
Microtubule Proteins
Growth
NIH 3T3 Cells
Diethylstilbestrol
Neoplasms
Oncogenes
Polymerization
KB Cells
Colchicine

Keywords

  • Estrogen
  • Indenestrol
  • KB cell
  • L1210 cell
  • MTT
  • NIH-3T3 cell

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells. / Oda, Taiko; Tanaka, Motohiro; Sasaki, Takuma.

In: Biological and Pharmaceutical Bulletin, Vol. 24, No. 10, 2001, p. 1142-1144.

Research output: Contribution to journalArticle

Oda, Taiko ; Tanaka, Motohiro ; Sasaki, Takuma. / Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells. In: Biological and Pharmaceutical Bulletin. 2001 ; Vol. 24, No. 10. pp. 1142-1144.
@article{c77eba049bfb4103b01611821a86b8f2,
title = "Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells",
abstract = "We have shown that synthetic and natural estrogens and related compounds inhibit the polymerization of microtubule proteins. In this study, cell growth inhibition by synthetic estrogens and their related compounds was examined by the MTT method using L1210, KB, and NIH-3T3 cells transformed with oncogenes, which are typical screening systems for carcinostatics. [(-)3R]Indenestrol B, a derivative of diethylstilbestrol (DES), which strongly inhibited the polymerization of microtubule proteins, also showed marked inhibition of the growth of KB cells and various oncogene-transformed NIH-3T3 cells. On the other hand, DES and indenestrol A markedly inhibited the growth of L1210 cells, indicating that these compounds exhibit cell-specific inhibitory effects on cell growth. Although the inhibition of cell growth by these compounds was not as strong as that by colchicine, the results clearly indicate the potential of these compounds as carcinostatics.",
keywords = "Estrogen, Indenestrol, KB cell, L1210 cell, MTT, NIH-3T3 cell",
author = "Taiko Oda and Motohiro Tanaka and Takuma Sasaki",
year = "2001",
doi = "10.1248/bpb.24.1142",
language = "English",
volume = "24",
pages = "1142--1144",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "10",

}

TY - JOUR

T1 - Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells

AU - Oda, Taiko

AU - Tanaka, Motohiro

AU - Sasaki, Takuma

PY - 2001

Y1 - 2001

N2 - We have shown that synthetic and natural estrogens and related compounds inhibit the polymerization of microtubule proteins. In this study, cell growth inhibition by synthetic estrogens and their related compounds was examined by the MTT method using L1210, KB, and NIH-3T3 cells transformed with oncogenes, which are typical screening systems for carcinostatics. [(-)3R]Indenestrol B, a derivative of diethylstilbestrol (DES), which strongly inhibited the polymerization of microtubule proteins, also showed marked inhibition of the growth of KB cells and various oncogene-transformed NIH-3T3 cells. On the other hand, DES and indenestrol A markedly inhibited the growth of L1210 cells, indicating that these compounds exhibit cell-specific inhibitory effects on cell growth. Although the inhibition of cell growth by these compounds was not as strong as that by colchicine, the results clearly indicate the potential of these compounds as carcinostatics.

AB - We have shown that synthetic and natural estrogens and related compounds inhibit the polymerization of microtubule proteins. In this study, cell growth inhibition by synthetic estrogens and their related compounds was examined by the MTT method using L1210, KB, and NIH-3T3 cells transformed with oncogenes, which are typical screening systems for carcinostatics. [(-)3R]Indenestrol B, a derivative of diethylstilbestrol (DES), which strongly inhibited the polymerization of microtubule proteins, also showed marked inhibition of the growth of KB cells and various oncogene-transformed NIH-3T3 cells. On the other hand, DES and indenestrol A markedly inhibited the growth of L1210 cells, indicating that these compounds exhibit cell-specific inhibitory effects on cell growth. Although the inhibition of cell growth by these compounds was not as strong as that by colchicine, the results clearly indicate the potential of these compounds as carcinostatics.

KW - Estrogen

KW - Indenestrol

KW - KB cell

KW - L1210 cell

KW - MTT

KW - NIH-3T3 cell

UR - http://www.scopus.com/inward/record.url?scp=0035159146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035159146&partnerID=8YFLogxK

U2 - 10.1248/bpb.24.1142

DO - 10.1248/bpb.24.1142

M3 - Article

VL - 24

SP - 1142

EP - 1144

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 10

ER -