D-Aspartate stimulation of testosterone synthesis in rat Leydig cells

Yoshiko Itoh, Hiroshi Homma, Jen Ai Lee, Kazuhiro Imai

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultured with D-aspartate for more than 3 h. The increased production of testosterone was well correlated with the amounts of d-aspartate incorporated into the Leydig cells, and L-cysteine sulfinic acid, an inhibitor of D-aspartate uptake, suppressed both testosterone production and intracellular D-aspartate levels. d-Aspartate therefore is presumably taken up into cells to increase steroidogenesis. Intracellular D-aspartate probably acts on cholesterol translocation into the inner mitochondrial membrane, the rate-limiting process in steroidogenesis. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)160-164
Number of pages5
JournalFEBS Letters
Volume444
Issue number2-3
DOIs
Publication statusPublished - 1999 Feb 12
Externally publishedYes

Fingerprint

D-Aspartic Acid
Leydig Cells
Testosterone
Rats
Aspartic Acid
Asparagine
Glutamate Receptors
Mitochondrial Membranes
Chorionic Gonadotropin
Cysteine
Glutamic Acid
Cultured Cells
Chemical activation
Cholesterol
Membranes

Keywords

  • D-Aspartate
  • Glutamate transporter
  • Human chorionic gonadotropin
  • Leydig cell
  • Steroidogenesis
  • Testosterone

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

D-Aspartate stimulation of testosterone synthesis in rat Leydig cells. / Itoh, Yoshiko; Homma, Hiroshi; Lee, Jen Ai; Imai, Kazuhiro.

In: FEBS Letters, Vol. 444, No. 2-3, 12.02.1999, p. 160-164.

Research output: Contribution to journalArticle

Itoh, Yoshiko ; Homma, Hiroshi ; Lee, Jen Ai ; Imai, Kazuhiro. / D-Aspartate stimulation of testosterone synthesis in rat Leydig cells. In: FEBS Letters. 1999 ; Vol. 444, No. 2-3. pp. 160-164.
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