D-Ser-containing humanin shows promotion of fibril formation

Kanehiro Hayashi, Jumpei Sasabe, Tomohiro Chiba, Sadakazu Aiso, Naoko Utsunomiya-Tate

Research output: Contribution to journalArticle

Abstract

Humanin (HN), a peptide of 24 amino acid residues, suppresses the neuronal cell death that is induced by the gene products of Alzheimer's disease. HN contains two Ser residues at positions 7 and 14. Because the proportion of d-Ser isomerized from l-Ser in proteins appears to increase as cellular organs age, we explored the structural effects of the isomerization of each Ser residue in HN. By using a thioflavin-T assay to detect fibril formation, we found that an HN derivative that contained two isomerized d-Ser residues had a greater tendency to form fibrils than did wild-type HN or HNs containing single d-Ser residues. A previous report showed that HN containing two d-Ser residues exerts neuroprotective activity. Our data, therefore, suggest that the fibril formation by HN that contains two d-Ser residues may promote HN neuroprotective activity.

Original languageEnglish
Pages (from-to)2293-2297
Number of pages5
JournalAmino Acids
Volume42
Issue number6
DOIs
Publication statusPublished - 2012 Jun

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humanin
Cell death
Isomerization
Assays
Alzheimer Disease
Cell Death
Genes
Derivatives
Amino Acids
Peptides
Proteins
thioflavin T

Keywords

  • α-Helix
  • β-Sheet
  • Circular dichroism
  • d-Ser
  • Humanin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

D-Ser-containing humanin shows promotion of fibril formation. / Hayashi, Kanehiro; Sasabe, Jumpei; Chiba, Tomohiro; Aiso, Sadakazu; Utsunomiya-Tate, Naoko.

In: Amino Acids, Vol. 42, No. 6, 06.2012, p. 2293-2297.

Research output: Contribution to journalArticle

Hayashi, Kanehiro ; Sasabe, Jumpei ; Chiba, Tomohiro ; Aiso, Sadakazu ; Utsunomiya-Tate, Naoko. / D-Ser-containing humanin shows promotion of fibril formation. In: Amino Acids. 2012 ; Vol. 42, No. 6. pp. 2293-2297.
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