Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons

Takahiro Fuchigami, Yutaka Sato, Yuya Tomita, Tetsuya Takano, Shin Ya Miyauchi, Yukinori Tsuchiya, Taro Saito, Kenichiro Kubo, Kazunori Nakajima, Mitsunori Fukuda, Mitsuharu Hattori, Shin Ichi Hisanaga

Research output: Contribution to journalArticle

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Abstract

Reelin-Dab1 signaling is indispensable for proper positioning of neurons in mammalian brain. Reelin is a glycoprotein secreted from Cajal-Reztuis cells in marginal zone of cerebral cortex, and its receptors are Apolipoprotein E receptor 2 (ApoER2) or very low density lipoprotein receptor (VLDLR) expressed on migrating neurons. When Reelin binds to ApoER2 or VLDLR, an adaptor protein Dab1 bound to the receptors undergoes Tyr phosphorylation that is essential for Reelin signaling. We reported previously that Cdk5-p35 phosphorylates Dab1 at Ser400 and Ser491 and the phosphorylation regulates its binding to CIN85, which is an SH3-containing multiadaptor protein involved in endocytic downregulation of receptor-tyrosine kinases. However, the interaction of CIN85 with Dab1 has not been addressed in neurons. We examined here a possibility that CIN85 has a role in Reelin signaling. We found nonpho-sphorylated Dab1-mediated colocalization of CIN85 with ApoER2. The colocalization of CIN85 with ApoER2 was increased in neurons stimulated with Reelin repeats 3-6, an active Reelin fragment. The stimulation recruited CIN85 to domains in plasma membrane where it colocalized with ApoER2 and Dab1 and then to EEA1-labeled early endosomes in the cytoplasm. In addition, Tyr phosphorylation of Dab1 strengthened the binding to CIN85. These results suggest that CIN85 participates in Reelin signaling through the binding to Dab1.

Original languageEnglish
Pages (from-to)410-424
Number of pages15
JournalGenes to Cells
Volume18
Issue number5
DOIs
Publication statusPublished - 2013 May

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Neurons
Phosphorylation
Proteins
Endosomes
Receptor Protein-Tyrosine Kinases
Cerebral Cortex
Glycoproteins
Cytoplasm
Down-Regulation
Cell Membrane
reelin receptor
VLDL receptor
low density lipoprotein receptor-related protein 8
Brain

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Fuchigami, T., Sato, Y., Tomita, Y., Takano, T., Miyauchi, S. Y., Tsuchiya, Y., ... Hisanaga, S. I. (2013). Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons. Genes to Cells, 18(5), 410-424. https://doi.org/10.1111/gtc.12045

Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons. / Fuchigami, Takahiro; Sato, Yutaka; Tomita, Yuya; Takano, Tetsuya; Miyauchi, Shin Ya; Tsuchiya, Yukinori; Saito, Taro; Kubo, Kenichiro; Nakajima, Kazunori; Fukuda, Mitsunori; Hattori, Mitsuharu; Hisanaga, Shin Ichi.

In: Genes to Cells, Vol. 18, No. 5, 05.2013, p. 410-424.

Research output: Contribution to journalArticle

Fuchigami, T, Sato, Y, Tomita, Y, Takano, T, Miyauchi, SY, Tsuchiya, Y, Saito, T, Kubo, K, Nakajima, K, Fukuda, M, Hattori, M & Hisanaga, SI 2013, 'Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons', Genes to Cells, vol. 18, no. 5, pp. 410-424. https://doi.org/10.1111/gtc.12045
Fuchigami, Takahiro ; Sato, Yutaka ; Tomita, Yuya ; Takano, Tetsuya ; Miyauchi, Shin Ya ; Tsuchiya, Yukinori ; Saito, Taro ; Kubo, Kenichiro ; Nakajima, Kazunori ; Fukuda, Mitsunori ; Hattori, Mitsuharu ; Hisanaga, Shin Ichi. / Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons. In: Genes to Cells. 2013 ; Vol. 18, No. 5. pp. 410-424.
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