Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells

Siro Simizu, Takayuki Teruya, Toshihiko Nogawa, Harumi Aono, Masashi Ueki, Masakazu Uramoto, Yuichi Kobayashi, Hiroyuki Osada

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1α, 25(OH)2D3 induced retinoic acid receptor-β and 1α, 25(OH)2 D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume383
Issue number4
DOIs
Publication statusPublished - 2009 Jun 12
Externally publishedYes

Keywords

  • Acute myeloid leukemia
  • All-trans retinoic acid
  • Cell cycle
  • Differentiation
  • HL-60
  • Natural product
  • Phoslactomycin
  • Protein phosphatase 2A
  • Vitamin D

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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