Decarboxylation and Racemization of Unnatural Compounds Using Artificial Enzymes Derived from Arylmalonate Decarboxylase

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Protein engineering has emerged as a powerful tool for engineering enzymes with new functions. We have been investigating an arylmalonate decarboxylase that acts on unnatural arylmalonates to give (R)-α-arylpropionates (profens). This chapter evaluates the potential use of directed evolution and rational design in completely changing the function of the decarboxylase to an artificial racemase and (S)-selective decarboxylase. The variants are useful biocatalysts for producing optically active profens.

Original languageEnglish
Title of host publicationGreen Biocatalysis
Publisherwiley
Pages59-70
Number of pages12
ISBN (Electronic)9781118828083
ISBN (Print)9781118822296
DOIs
Publication statusPublished - 2016 May 13

Keywords

  • Artificial enzyme
  • Arylmalonate decarboxylase
  • Directed evolution
  • Profen

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)

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