Deciphering the landscape of host barriers to Listeria monocytogenes infection

Ting Zhang, Sören Abel, Pia Abelzur Wiesch, Jumpei Sasabe, Brigid M. Davis, Darren E. Higgins, Matthew K. Waldor

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Listeria monocytogenes is a common food-borne pathogen that can disseminate from the intestine and infect multiple organs. Here, we used sequence tag-based analysis ofmicrobial populations (STAMP) to investigate L. monocytogenes population dynamics during infection. We created a genetically barcoded library of murinized L. monocytogenes and then used deep sequencing to track the pathogen's dissemination routes and quantify its founding population (Nb) sizes in different organs. We found that the pathogen disseminates from the gastrointestinal tract to distal sites through multiple independent routes and that Nb sizes vary greatly among tissues, indicative of diverse host barriers to infection. Unexpectedly, comparative analyses of sequence tags revealed that fecally excreted organisms are largely derived from the very small number of L. monocytogenes cells that colonize the gallbladder. Immune depletion studies suggest that distinct innate immune cells restrict the pathogen's capacity to establish replicative niches in the spleen and liver. Finally, studies in germ-free mice suggest that the microbiota plays a critical role in the development of the splenic, but not the hepatic, barriers that prevent L. monocytogenes from seeding these organs. Collectively, these observations illustrate the potency of the STAMP approach to decipher the impact of host factors on population dynamics of pathogens during infection.

Original languageEnglish
Pages (from-to)6334-6339
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number24
DOIs
Publication statusPublished - 2017 Jun 13

Fingerprint

Listeriosis
Listeria monocytogenes
Population Dynamics
Infection
High-Throughput Nucleotide Sequencing
Liver
Microbiota
Population Density
Gallbladder
Population
Libraries
Intestines
Sequence Analysis
Gastrointestinal Tract
Spleen
Food

Keywords

  • Listeria monocytogenes
  • Pathogen dissemination
  • Pathogen transmission
  • Population dynamics
  • Stamp

ASJC Scopus subject areas

  • General

Cite this

Deciphering the landscape of host barriers to Listeria monocytogenes infection. / Zhang, Ting; Abel, Sören; Wiesch, Pia Abelzur; Sasabe, Jumpei; Davis, Brigid M.; Higgins, Darren E.; Waldor, Matthew K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 24, 13.06.2017, p. 6334-6339.

Research output: Contribution to journalArticle

Zhang, Ting ; Abel, Sören ; Wiesch, Pia Abelzur ; Sasabe, Jumpei ; Davis, Brigid M. ; Higgins, Darren E. ; Waldor, Matthew K. / Deciphering the landscape of host barriers to Listeria monocytogenes infection. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 24. pp. 6334-6339.
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