Decrease in expression of the master operon of flagellin synthesis in a dnaA46 mutant of Escherichia coli

Tohru Mizushima, Rimiko Koyanagi, Tsutomu Katayama, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We examined the expression of the fliC, fliA and flhD genes in the dnaA46 mutant. In Northern blot analysis, the amounts of fliC, fliA, and flhD mRNA decreased in the dnaA46 mutant growing at 37°C but not at 28 °C. The promoter activity of each gene also decreased in this mutant at 37°C, but not at 28°C. The decrease in expression of the flhD gene was not related to the cAMP-catabolite activator protein (CAP) pathway. We tentatively conclude that DnaA protein is involved in the expression of the flhD gene by a mechanism independent of the cAMP-CAP pathway.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number4
DOIs
Publication statusPublished - 1997 Apr

Keywords

  • DnaA protein
  • cAMP catabolite activator protein pathway
  • flagellation
  • flhD gene
  • promoter activity
  • transcription

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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