Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli

Tohru Mizushima, Junko Tanino, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitive dnaA mutants, including dnaA5 and dnaA46 mutants. The linking number of DNA prepared from dnaA mutants growing at 37°C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28°C. Complementation analysis with a plasmid containing the wild type dnaA gene and phage P1-mediated transduction confirmed that mutations in the dnaA gene were responsible for the decrease in the linking number of DNA.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number1
DOIs
Publication statusPublished - 1996 Jan 5

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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