Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli

Tohru Mizushima, Junko Tanino, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitive dnaA mutants, including dnaA5 and dnaA46 mutants. The linking number of DNA prepared from dnaA mutants growing at 37°C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28°C. Complementation analysis with a plasmid containing the wild type dnaA gene and phage P1-mediated transduction confirmed that mutations in the dnaA gene were responsible for the decrease in the linking number of DNA.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number1
DOIs
Publication statusPublished - 1996 Jan 5
Externally publishedYes

Fingerprint

Escherichia coli
Plasmids
Genes
Bacteriophage P1
Bacteriophages
Agar Gel Electrophoresis
DNA
Chloroquine
Electrophoresis
Sepharose
Gels
Mutation
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli. / Mizushima, Tohru; Tanino, Junko; Miki, Takeyoshi; Sekimizu, Kazuhisa.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 1, 05.01.1996, p. 137-141.

Research output: Contribution to journalArticle

Mizushima, Tohru ; Tanino, Junko ; Miki, Takeyoshi ; Sekimizu, Kazuhisa. / Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 218, No. 1. pp. 137-141.
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