Decrease of transplantability by the immunopotentiators, OK-432 and interleukin-2: Experiments on a human hepatoma cell line in nude mice

Hidetsugu Saito, Toshio Morizane, Yasutaka Inagaki, Naoki Kumagai, Tetsu Watanabe, Tatsuya Nakamura, Itsurou Satoh, Kentarou Sawaguchi, Naoto Iwabuchi, Kanji Tsuchimoto, Masaharu Tsuchiya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The relationship between nonspecific cytotoxic activity of spleen cells and the resistance against the graft challenge of a human hepatoma cell line (HCC-M) was investigated in nude mice. Two administrations of an immunopotentiator, OK-432 or human interleukin-2, prior to the subcutaneous inoculation of HCC-M cells, which was performed 24 h after the last administration, significantly inhibited the tumor development in terms of rate of tumor take and tumor size. This effect was abrogated by simultaneous administration of an anti-asialo GM1 (ASGM1) antiserum. There was a significant inverse correlation between tumor volume and spleen cell cytotoxicity which was determined at the time of HCC-M cell inoculation against a YAC-1 or HCC-M target. Spleen cell cytotoxicity enhanced by these immunopotentiators could not completely be abolished by in vitro treatment with ASGM1 and complement. This result suggests that effector cells of the enhanced cytotoxicity consist of heterogeneous cells including both ASGM1+ natural killer cells and other nonselective cytotoxic cells. These results suggest that nonspecific cytotoxic cells play crucial roles in the resistance against tumor cell challenge and that the total level of cytotoxic activity of these cells at the time of tumor cell challenge is a key factor which determines tumor development.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalEuropean Journal of Cancer and Clinical Oncology
Volume25
Issue number1
DOIs
Publication statusPublished - 1989 Jan

    Fingerprint

ASJC Scopus subject areas

  • Oncology

Cite this

Saito, H., Morizane, T., Inagaki, Y., Kumagai, N., Watanabe, T., Nakamura, T., Satoh, I., Sawaguchi, K., Iwabuchi, N., Tsuchimoto, K., & Tsuchiya, M. (1989). Decrease of transplantability by the immunopotentiators, OK-432 and interleukin-2: Experiments on a human hepatoma cell line in nude mice. European Journal of Cancer and Clinical Oncology, 25(1), 79-89. https://doi.org/10.1016/0277-5379(89)90054-0