Decrease of vascular endothelial growth factor in macrophages from long-term smokers

K. Nagai, Tomoko Betsuyaku, Y. Ito, Y. Nasuhara, M. Nishimura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF), a survival factor for endothelial cells and a promoter of anglogenesis, is reportedly expressed in alveolar macrophages (AMs). To investigate whether long-term smoking with age affects VEGF expression in AMs, bronchoalveolar lavage (BAL) was performed on 18 young and 23 older volunteers with various smoking histories. The expressions of VEGF and its functional receptor, fms-like tyrosine kinase (Flt)-1, were quantified in AMs by real-time RT-PCR and, further, the level of VEGF in BAL fluid was determined by ELISA. VEGF mRNA in AMs demonstrated a 1.8-fold reduction in current smokers compared with nonsmokers in older subjects and, furthermore, a 1.5-fold downregulation in those with emphysema, although there was no difference between current smokers and nonsmokers among the young subjects. The downregulation in total VEGF mRNA was supported by the substantial reduction of VEGF121 and VEGF165 isoforms. However, in contrast, Flt-1 mRNA did not differ within the older groups, whereas it was upregulated in young current smokers compared with age-matched nonsmokers. VEGF in BAL fluid is significantly decreased in current smokers compared with nonsmokers, regardless of their age. In conclusion, these data imply that the biological availability of vascular endothelial growth factor in alveolar macrophages is impaired in older current smokers with long-term smoking histories.

Original languageEnglish
Pages (from-to)626-633
Number of pages8
JournalEuropean Respiratory Journal
Volume25
Issue number4
DOIs
Publication statusPublished - 2005 Apr
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Macrophages
Alveolar Macrophages
Vascular Endothelial Growth Factor Receptor-1
Smoking
Bronchoalveolar Lavage Fluid
Messenger RNA
Down-Regulation
Emphysema
Bronchoalveolar Lavage
Biological Availability
Real-Time Polymerase Chain Reaction
Volunteers
Protein Isoforms
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Survival

Keywords

  • Ageing
  • Apoptosis
  • Bronchoalveolar lavage
  • Chronic obstructive pulmonary disease
  • Emphysema
  • Smoking

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Decrease of vascular endothelial growth factor in macrophages from long-term smokers. / Nagai, K.; Betsuyaku, Tomoko; Ito, Y.; Nasuhara, Y.; Nishimura, M.

In: European Respiratory Journal, Vol. 25, No. 4, 04.2005, p. 626-633.

Research output: Contribution to journalArticle

Nagai, K. ; Betsuyaku, Tomoko ; Ito, Y. ; Nasuhara, Y. ; Nishimura, M. / Decrease of vascular endothelial growth factor in macrophages from long-term smokers. In: European Respiratory Journal. 2005 ; Vol. 25, No. 4. pp. 626-633.
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