TY - JOUR
T1 - Decreased expression of the amplified mdr1 gene in revertants of multidrug-resistant human myelogenous leukemia K562 occurs without loss of amplified DNA
AU - Sugimoto, Y.
AU - Roninson, I. B.
AU - Tsuruo, T.
PY - 1987
Y1 - 1987
N2 - Amplification and increased expression of the mdr1 gene associated with multidrug resistance in human tumors were found in multidrug-resistant sublines of human myelogenous leukemia K562 selected with vincristine (K562/VCR) or adriamycin (K562/ADM). In two revertant cell lines of K562/ADM, amplification of the mdr1 gene was maintained at the same level as in K562/ADM, but expression of the 4,5-kilobase mdr1 mRNA was greatly decreased, indicating that amplified genes may be inactivated at the level of transcription without a corresponding loss of amplified DNA.
AB - Amplification and increased expression of the mdr1 gene associated with multidrug resistance in human tumors were found in multidrug-resistant sublines of human myelogenous leukemia K562 selected with vincristine (K562/VCR) or adriamycin (K562/ADM). In two revertant cell lines of K562/ADM, amplification of the mdr1 gene was maintained at the same level as in K562/ADM, but expression of the 4,5-kilobase mdr1 mRNA was greatly decreased, indicating that amplified genes may be inactivated at the level of transcription without a corresponding loss of amplified DNA.
UR - http://www.scopus.com/inward/record.url?scp=0023638816&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023638816&partnerID=8YFLogxK
U2 - 10.1128/MCB.7.12.4549
DO - 10.1128/MCB.7.12.4549
M3 - Article
C2 - 3481435
AN - SCOPUS:0023638816
SN - 0270-7306
VL - 7
SP - 4549
EP - 4552
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 12
ER -