Decreased insulin-like growth factor I receptor expression and function in immortalized African Pygmy T cells

Yutaka Hattori, Juan Carlos Vera, Coralia I. Rivas, Noelle Bersch, Robert C. Bailey, Mitchell E. Geffner, David W. Golde

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Efe Pygmies of northeast Zaire have the shortest mean adult stature of any population on earth. Although various alterations in the GH/insulin-like growth factor I (IGF-I) axis have been suggested, the basis for short stature in the Pygmy is unknown. We previously described IGF-I unresponsiveness in a T lymphoblast cell line derived from an Efe Pygmy, and studies in five additional lines have confirmed severe IGF-I resistance in these cells. We have now performed experiments to determine the molecular basis for the IGF- I resistance in these cells. We found markedly decreased cell surface expression of IGF-I receptors with normal ligand binding affinity. The Pygmy IGF-I receptors were not autophosphorylated and did not transmit a signal in response to physiological concentrations of IGF-I. There was a substantially decreased level of IGF-I receptor messenger ribonucleic acid in the Pygmy cells with a normal messenger ribonucleic acid half-life. The nucleotide sequence of the full-length IGF receptor complementary DNA in Pygmy 1 showed no significant variation. These results indicate decreased IGF-I receptor gene transcription and IGF-I receptor signaling as the primary variation in the Pygmy cell lines. The findings point to the IGF-I receptor as the locus governing short stature in the African Pygmy and suggest that human stature may be genetically controlled by expression of the IGF-I receptor.

Original languageEnglish
Pages (from-to)2257-2263
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume81
Issue number6
DOIs
Publication statusPublished - 1996
Externally publishedYes

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IGF Type 1 Receptor
T-cells
Insulin-Like Growth Factor I
T-Lymphocytes
Cells
RNA
Democratic Republic of the Congo
Cell Line
Transcription
Half-Life
Nucleotides
Complementary DNA
Genes
Earth (planet)
Ligands
Population

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Decreased insulin-like growth factor I receptor expression and function in immortalized African Pygmy T cells. / Hattori, Yutaka; Vera, Juan Carlos; Rivas, Coralia I.; Bersch, Noelle; Bailey, Robert C.; Geffner, Mitchell E.; Golde, David W.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 81, No. 6, 1996, p. 2257-2263.

Research output: Contribution to journalArticle

Hattori, Yutaka ; Vera, Juan Carlos ; Rivas, Coralia I. ; Bersch, Noelle ; Bailey, Robert C. ; Geffner, Mitchell E. ; Golde, David W. / Decreased insulin-like growth factor I receptor expression and function in immortalized African Pygmy T cells. In: Journal of Clinical Endocrinology and Metabolism. 1996 ; Vol. 81, No. 6. pp. 2257-2263.
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