Deep anesthesia worsens outcome of rats with inflammatory responses

Kei Inoue, Takeshi Suzuki, Toru Igarashi, Shizuka Minamishima, Hiroyuki Seki, Shizuko Kosugi, Nobuyuki Katori, Hiroshi Morisaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: We tested the hypothesis that deep anesthesia with sevoflurane, known as a potent immunomodulator, for 4 h would worsen the 24-h outcomes of rats through modulation of the inflammatory responses. Methods: Forty-nine male Wistar rats, administered low dose of lipopolysaccharide (0.5 mg/kg) intravenously to elicit moderate inflammatory responses mimicked mild surgical stress, underwent one minimum alveolar concentration (MAC) or 2 MAC sevoflurane anesthesia for 4 h. The 24-h survival rate, arterial blood gases, plasma interleukin (IL)-6 and tumor necrosis factor (TNF)-α concentrations, and rate of T lymphocyte apoptosis in spleen were evaluated. We further examined the effects of hypotension and TNF-α discharge on the survival rate. Results: The survival rate in 2 MAC group was significantly lower accompanied with decreased base excess and increased level of cytokines (IL-6, TNF-α) compared to 1 MAC group. The apoptosis rate did not differ between the two groups. Neither norepinephrine infusion to restore hypotension nor administration of anti-TNF-α antibody improved the outcome in the 2 MAC group. Conclusions: Deep anesthesia with sevoflurane even for a short-term period augments the release of inflammatory cytokines evoked by inflammatory insults like surgical stress, impairs the acid–base balance, and subsequently deteriorates the outcomes.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInflammation Research
DOIs
Publication statusAccepted/In press - 2016 Mar 21

Fingerprint

Anesthesia
Tumor Necrosis Factor-alpha
Hypotension
Interleukin-6
Apoptosis
Cytokines
Immunologic Factors
Lipopolysaccharides
Wistar Rats
Norepinephrine
Spleen
Gases
T-Lymphocytes
Antibodies
sevoflurane

Keywords

  • Cytokine
  • Inflammation
  • Outcome
  • Sevoflurane

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

Deep anesthesia worsens outcome of rats with inflammatory responses. / Inoue, Kei; Suzuki, Takeshi; Igarashi, Toru; Minamishima, Shizuka; Seki, Hiroyuki; Kosugi, Shizuko; Katori, Nobuyuki; Morisaki, Hiroshi.

In: Inflammation Research, 21.03.2016, p. 1-9.

Research output: Contribution to journalArticle

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AU - Suzuki, Takeshi

AU - Igarashi, Toru

AU - Minamishima, Shizuka

AU - Seki, Hiroyuki

AU - Kosugi, Shizuko

AU - Katori, Nobuyuki

AU - Morisaki, Hiroshi

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N2 - Objective: We tested the hypothesis that deep anesthesia with sevoflurane, known as a potent immunomodulator, for 4 h would worsen the 24-h outcomes of rats through modulation of the inflammatory responses. Methods: Forty-nine male Wistar rats, administered low dose of lipopolysaccharide (0.5 mg/kg) intravenously to elicit moderate inflammatory responses mimicked mild surgical stress, underwent one minimum alveolar concentration (MAC) or 2 MAC sevoflurane anesthesia for 4 h. The 24-h survival rate, arterial blood gases, plasma interleukin (IL)-6 and tumor necrosis factor (TNF)-α concentrations, and rate of T lymphocyte apoptosis in spleen were evaluated. We further examined the effects of hypotension and TNF-α discharge on the survival rate. Results: The survival rate in 2 MAC group was significantly lower accompanied with decreased base excess and increased level of cytokines (IL-6, TNF-α) compared to 1 MAC group. The apoptosis rate did not differ between the two groups. Neither norepinephrine infusion to restore hypotension nor administration of anti-TNF-α antibody improved the outcome in the 2 MAC group. Conclusions: Deep anesthesia with sevoflurane even for a short-term period augments the release of inflammatory cytokines evoked by inflammatory insults like surgical stress, impairs the acid–base balance, and subsequently deteriorates the outcomes.

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