Defective smooth muscle development in qkl-deficient mice

Zhenghua Li, Nobuyuki Takakura, Yuichi Oike, Takanobu Imanaka, Kimi Araki, Toshio Suda, Tadashi Kaname, Tatsuya Kondo, Kuniya Abe, Ken Ichi Yamamura

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The qkl gene encodes an RNA binding protein which was identified as a candidate for the classical neurologic mutation, qkv. Although qkl is involved in glial cell differentiation in mice, qkl homologues in other species play important roles in various developmental processes. Here, we show a novel function of qkl in smooth muscle cell differentiation during embryonic blood vessel formation, qkl null embryos died between embryonic day 9.5 and 10.5. Embryonic day 9.5 qkl null embryos showed a lack of large vitelline vessels in the yolk sacs, kinky neural tubes, pericardial effusion, open neural tubes and incomplete embryonic turning. Using X-gal and immunohistochemical staining, qkl is first shown to be expressed in endothelial cells and smooth muscle cells. Analyses of qkl null embryos in vivo and in vitro revealed that the vitelline artery was too thin to connect properly to the yolk sac, thereby preventing remodeling of the yolk sac vasculature, and that the vitelline vessel was deficient in smooth muscle cells. Addition of QKI and platelet-endothelial cell adhesion molecule-1 positive cells to an in vitro para-aortic splanchnopleural culture of qkl null embryos rescued the vascular remodeling deficit. These data suggest that QKI protein has a critical regulatory role in smooth muscle cell development, and that smooth muscle cells play an important role in inducing vascular remodeling.

Original languageEnglish
Pages (from-to)449-462
Number of pages14
JournalDevelopment Growth and Differentiation
Volume45
Issue number5-6
DOIs
Publication statusPublished - 2003 Oct

Fingerprint

Muscle Development
Smooth Muscle Myocytes
Smooth Muscle
Yolk Sac
Embryonic Structures
Neural Tube
Cell Differentiation
CD31 Antigens
RNA-Binding Proteins
Pericardial Effusion
Neuroglia
Nervous System
Blood Vessels
Endothelial Cells
Arteries
Staining and Labeling
Mutation
Genes
Proteins
Vascular Remodeling

Keywords

  • Blood vessel
  • Embryonic lethality
  • Knockout mouse
  • Quaking
  • Smooth muscle cell

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Li, Z., Takakura, N., Oike, Y., Imanaka, T., Araki, K., Suda, T., ... Yamamura, K. I. (2003). Defective smooth muscle development in qkl-deficient mice. Development Growth and Differentiation, 45(5-6), 449-462. https://doi.org/10.1111/j.1440-169X.2003.00712.x

Defective smooth muscle development in qkl-deficient mice. / Li, Zhenghua; Takakura, Nobuyuki; Oike, Yuichi; Imanaka, Takanobu; Araki, Kimi; Suda, Toshio; Kaname, Tadashi; Kondo, Tatsuya; Abe, Kuniya; Yamamura, Ken Ichi.

In: Development Growth and Differentiation, Vol. 45, No. 5-6, 10.2003, p. 449-462.

Research output: Contribution to journalArticle

Li, Z, Takakura, N, Oike, Y, Imanaka, T, Araki, K, Suda, T, Kaname, T, Kondo, T, Abe, K & Yamamura, KI 2003, 'Defective smooth muscle development in qkl-deficient mice', Development Growth and Differentiation, vol. 45, no. 5-6, pp. 449-462. https://doi.org/10.1111/j.1440-169X.2003.00712.x
Li, Zhenghua ; Takakura, Nobuyuki ; Oike, Yuichi ; Imanaka, Takanobu ; Araki, Kimi ; Suda, Toshio ; Kaname, Tadashi ; Kondo, Tatsuya ; Abe, Kuniya ; Yamamura, Ken Ichi. / Defective smooth muscle development in qkl-deficient mice. In: Development Growth and Differentiation. 2003 ; Vol. 45, No. 5-6. pp. 449-462.
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